Linked Life Data

1718943

Source:http://linkedlifedata.com/resource/pubmed/id/1718943

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1991-12-2
pubmed:abstractText
Local anesthetics are known to reduce the level of OmpF and increase the synthesis of OmpC in the outer membrane of Escherichia coli K-12. It has been shown that the anesthetics procaine and phenethyl alcohol (PEA) act at the transcriptional level for ompF and ompC and that in the case of procaine, its action is dependent on EnvZ, the membrane-bound signal transducer required for ompF and ompC expression. In an effort to further understand how anesthetics regulate ompF and ompC expression, we have analyzed the DNA binding properties of OmpR (the transcriptional activator protein for ompF and ompC genes) from cells treated with procaine or PEA. Treatment of a wild-type cell with either anesthetic converted OmpR from a low-affinity DNA binding form to a high-affinity DNA binding form. The change in DNA binding affinity was correlated with alterations in outer membrane porin profiles and could occur in the absence of protein synthesis. A strain lacking EnvZ was unable to respond to procaine to produce either the shift in the OmpR DNA binding property or cause any change in the outer membrane porin profile. PEA treatment was also dependent on EnvZ for the alteration in the OmpR DNA binding property, but it could induce ompC expression in the absence of EnvZ. Further studies suggest that the amino-terminal region of EnvZ is responsible for the procaine signalling. Our results indicate that procaine and PEA regulate ompF and ompC expression by modifying the DNA binding properties of OmpR through EnvZ signal transduction.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-115844, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-1700256, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-1850414, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-1964084, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2045368, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2157353, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2160945, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2204430, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2277042, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2407554, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2476847, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2553720, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2557454, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2558046, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2580220, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2651863, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2656631, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2656684, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2656704, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2663643, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2668281, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2668953, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2674113, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2823064, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2824492, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2846509, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-2997120, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3010044, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3011737, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3023382, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3056929, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3275653, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-328490, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3294816, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-377291, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-3843705, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-4555469, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-5335963, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-6275366, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-6304323, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-6311806, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-6434513, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-6816944, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-6994810, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-7021525, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-7021856, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-7024253, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-781293, http://linkedlifedata.com/resource/pubmed/commentcorrection/1718943-89196
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
173
pubmed:geneSymbol
envZ, ompC, ompF, ompR
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6882-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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