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rdf:type |
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lifeskim:mentions |
umls-concept:C0003069,
umls-concept:C0030016,
umls-concept:C0031764,
umls-concept:C0151956,
umls-concept:C0205307,
umls-concept:C0242606,
umls-concept:C0740009,
umls-concept:C0870432,
umls-concept:C1521828,
umls-concept:C1555465,
umls-concept:C1705417
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pubmed:issue |
2
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pubmed:dateCreated |
2007-2-7
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pubmed:abstractText |
Ferredoxin-NADP(H) reductase (FNR) catalyzes the last step of photosynthetic electron transport in chloroplasts, driving electrons from reduced ferredoxin to NADP+. This reaction is rate limiting for photosynthesis under a wide range of illumination conditions, as revealed by analysis of plants transformed with an antisense version of the FNR gene. To investigate whether accumulation of this flavoprotein over wild-type levels could improve photosynthetic efficiency and growth, we generated transgenic tobacco (Nicotiana tabacum) plants expressing a pea (Pisum sativum) FNR targeted to chloroplasts. The alien product distributed between the thylakoid membranes and the chloroplast stroma. Transformants grown at 150 or 700 micromol quanta m(-2) s(-1) displayed wild-type phenotypes regardless of FNR content. Thylakoids isolated from plants with a 5-fold FNR increase over the wild type displayed only moderate stimulation (approximately 20%) in the rates of electron transport from water to NADP+. In contrast, when donors of photosystem I were used to drive NADP+ photoreduction, the activity was 3- to 4-fold higher than the wild-type controls. Plants expressing various levels of FNR (from 1- to 3.6-fold over the wild type) failed to show significant differences in CO2 assimilation rates when assayed over a range of light intensities and CO2 concentrations. Transgenic lines exhibited enhanced tolerance to photooxidative damage and redox-cycling herbicides that propagate reactive oxygen species. The results suggest that photosynthetic electron transport has several rate-limiting steps, with FNR catalyzing just one of them.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-10712553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-11035018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-11483610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-11581664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-11844106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-11844783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-12207018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-12709048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-12887584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-1397306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-14572660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-14645726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-15078333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-15134648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-15894798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-16549675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-16666920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-16668627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-16956929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-17835129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-2101692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-3016666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-603028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-6480592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-6736031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-7236641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-7370237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-7635836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-7919218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-9085572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-9414570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189326-9628012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0032-0889
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pubmed:author |
pubmed-author:CarrilloNéstorN,
pubmed-author:HajirezaeiMohammad-RezaMR,
pubmed-author:LodeyroAnabellaA,
pubmed-author:PalatnikJavier FJF,
pubmed-author:PeiskerMartinM,
pubmed-author:PoliHugo OHO,
pubmed-author:RodriguezRamiro ERE,
pubmed-author:TognettiVanesa BVB,
pubmed-author:TschierschHenningH,
pubmed-author:ValleEstela MEM,
pubmed-author:ZurbriggenMatiasM
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pubmed:issnType |
Print
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
639-49
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pubmed:dateRevised |
2010-9-16
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pubmed:meshHeading |
pubmed-meshheading:17189326-Carbon Dioxide,
pubmed-meshheading:17189326-Chloroplasts,
pubmed-meshheading:17189326-Ferredoxin-NADP Reductase,
pubmed-meshheading:17189326-Gene Expression Regulation, Plant,
pubmed-meshheading:17189326-Herbicides,
pubmed-meshheading:17189326-Light,
pubmed-meshheading:17189326-Oxidative Stress,
pubmed-meshheading:17189326-Paraquat,
pubmed-meshheading:17189326-Peas,
pubmed-meshheading:17189326-Photosynthesis,
pubmed-meshheading:17189326-Plants, Genetically Modified,
pubmed-meshheading:17189326-Tobacco
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pubmed:year |
2007
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pubmed:articleTitle |
Transgenic tobacco plants overexpressing chloroplastic ferredoxin-NADP(H) reductase display normal rates of photosynthesis and increased tolerance to oxidative stress.
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pubmed:affiliation |
Instituto de Biología Molecular y Celular de Rosario, División Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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