|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2007-2-19
|
|
pubmed:abstractText |
The transport of ammonia/ammonium is fundamental to nitrogen metabolism in all forms of life. So far, no clear picture has emerged as to whether a protein channel is capable of transporting exclusively neutral NH(3) while excluding H(+) and NH(4)(+). Our research is the first stoichiometric study to show the selective transport of NH(3) by a membrane channel. The purified water channel protein aquaporin-8 was reconstituted into planar bilayers, and the exclusion of NH(4)(+) or H(+) was established by ensuring a lack of current under voltage clamp conditions. The single channel water permeability coefficient of 1.2 x 10(-14) cm(3)/subunit/s was established by imposing an osmotic gradient across reconstituted planar bilayers, and resulting minute changes in ionic concentration close to the membrane surface were detected. It is more than 2-fold smaller than the single channel ammonia permeability (2.7 x 10(-14) cm(3)/subunit/s) that was derived by establishing a transmembrane ammonium concentration gradient and measuring the resulting concentration increases adjacent to the membrane. This permeability ratio suggests that electrically silent ammonia transport may be the main function of AQP8.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-10647010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11278499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11410596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11493683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11585847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11700553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11773613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11821433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-11909995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-12740433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-14701836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15034178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15340377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15358535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15361618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15551866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15563598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15572441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15749715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15850456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15948717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-15988592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16214888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16273393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16440338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16446503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16499477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16563831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16624821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16712124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16870726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-16910683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-2417221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-6269143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-8369403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-8580325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-9045617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-9083675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-9129821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17189259-9388476
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
23
|
|
pubmed:volume |
282
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5296-301
|
|
pubmed:dateRevised |
2011-7-26
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Fast and selective ammonia transport by aquaporin-8.
|
|
pubmed:affiliation |
Institut für Biophysik, Kepler Universität Linz, Altenbergerstrasse 69, A-4040 Linz, Austria.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
