Source:http://linkedlifedata.com/resource/pubmed/id/17182554
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2006-12-21
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pubmed:abstractText |
IL-23 plays crucial roles in both immunity against pathogens and autoimmunity against self. Although it is well recognized that IL-23 expression is restricted to the myeloid lineage and is tightly regulated at the transcriptional level, the nature of transcription factors required for IL-23 expression is poorly understood. We report, in this study, that murine dendritic cells deficient in c-Rel, a member of the NF-kappaB family, are severely compromised in their ability to transcribe the p19 gene, one of the two genes that encode the IL-23 protein. The p19 gene promoter contains three putative NF-kappaB binding sites, two of which can effectively bind c-Rel as determined by chromatin immunoprecipitation and EMSA. Unexpectedly, mutation of either of these two c-Rel binding sites completely abolished the p19 promoter activity induced by five TLRs (2, 3, 4, 6, and 9) and four members of the NF-kappaB family (c-Rel, p65, p100, and p105). Based on these observations, we conclude that c-Rel controls IL-23 p19 gene expression through two kappaB sites in the p19 promoter, and propose a c-Rel-dependent enhanceosome model for p19 gene activation.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
186-91
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17182554-Animals,
pubmed-meshheading:17182554-Base Sequence,
pubmed-meshheading:17182554-Chromatin Immunoprecipitation,
pubmed-meshheading:17182554-Dendritic Cells,
pubmed-meshheading:17182554-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:17182554-Gene Expression Regulation,
pubmed-meshheading:17182554-Interleukin-23 Subunit p19,
pubmed-meshheading:17182554-Mice,
pubmed-meshheading:17182554-Mice, Inbred C57BL,
pubmed-meshheading:17182554-Molecular Sequence Data,
pubmed-meshheading:17182554-NF-kappa B,
pubmed-meshheading:17182554-Promoter Regions, Genetic,
pubmed-meshheading:17182554-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:17182554-Toll-Like Receptors,
pubmed-meshheading:17182554-Transcriptional Activation
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pubmed:year |
2007
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pubmed:articleTitle |
Essential roles of c-Rel in TLR-induced IL-23 p19 gene expression in dendritic cells.
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pubmed:affiliation |
Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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