Linked Life Data

17181472

Source:http://linkedlifedata.com/resource/pubmed/id/17181472

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-12-21
pubmed:abstractText
Knowing that a specific protein is present within a cell provides little insight into its function. In a study by Schubert and colleagues, the investigators present a multidimensional method that utilizes fluorescence microscopy and automated antibody introduction and detection, which is potentially capable of localizing hundreds of proteins within individual cells. The method, referred to as multiepitope-ligand cartography, is validated in the analysis of cell-surface receptors in peripheral mononuclear blood cells, and then used to map protein complexes in a series of disease models, including psoriasis and chronic constriction injury. Within each experiment, the locales of each protein are presented in a binary format and the data are interpreted to recognize specific proteins that control the topology of the protein network. The hope is that by identifying partnerships between proteins and those proteins that are most responsible for these interactions, novel diagnostic features and therapeutic targets can be established.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Dec
pubmed:issn
1744-8387
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
581-3
pubmed:year
2006
pubmed:articleTitle
Multiplexed immunofluorescence microscopy for the interrogation of cellular protein complexes.
pubmed:affiliation
National Cancer Institute at Frederick, Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick Inc., Frederick, MD 21702-1201, USA. mzhou@ncifcrf.gov
pubmed:publicationType
Journal Article, Comment