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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 2
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pubmed:dateCreated |
2007-1-11
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pubmed:abstractText |
Ena/VASP proteins negatively regulate cell motility and contribute to repulsion from several guidance cues; however, there is currently no evidence for a role downstream of Eph receptors. Eph receptors mediate repulsion from ephrins at sites of intercellular contact during several developmental migrations. For example, the expression of ephrin-Bs in posterior halves of somites restricts neural crest cell migration to the anterior halves. Here we show that ephrin-B2 destabilises neural crest cell lamellipodia when presented in a substrate-bound or soluble form. Our timelapse studies show that repulsive events are associated with the rearward collapse and subsequent loss of lamellipodia as membrane ruffles. We hypothesise that Ena/VASP proteins contribute to repulsion from ephrins by destabilising cellular protrusions and show that Ena/VASP-deficient fibroblasts exhibit reduced repulsion from both ephrin-A and ephrin-B stripes compared to wild-type controls. Moreover, when EphB4 and ephrin-B2 were expressed in neighbouring Swiss 3T3 fibroblasts, VASP and Mena co-accumulated with activated Eph receptors at protrusions formed by EphB4-expressing cells. Sequestration of Ena/VASP proteins away from the periphery of these cells inhibited Eph receptor internalisation, a process that facilitates repulsion. Our results suggest that Ena/VASP proteins regulate ephrin-induced Eph receptor signalling events, possibly by destabilising lamellipodial protrusions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ephrin-A5,
http://linkedlifedata.com/resource/pubmed/chemical/Ephrin-B2,
http://linkedlifedata.com/resource/pubmed/chemical/Ephrins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Phalloidine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Eph Family
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
289-98
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17179204-3T3 Cells,
pubmed-meshheading:17179204-Animals,
pubmed-meshheading:17179204-Cell Line, Transformed,
pubmed-meshheading:17179204-Cells, Cultured,
pubmed-meshheading:17179204-DNA-Binding Proteins,
pubmed-meshheading:17179204-Ephrin-A5,
pubmed-meshheading:17179204-Ephrin-B2,
pubmed-meshheading:17179204-Ephrins,
pubmed-meshheading:17179204-Fibroblasts,
pubmed-meshheading:17179204-Fluorescein-5-isothiocyanate,
pubmed-meshheading:17179204-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:17179204-Fluorescent Dyes,
pubmed-meshheading:17179204-Green Fluorescent Proteins,
pubmed-meshheading:17179204-Ligands,
pubmed-meshheading:17179204-Mice,
pubmed-meshheading:17179204-Mice, Knockout,
pubmed-meshheading:17179204-Microscopy, Video,
pubmed-meshheading:17179204-Neural Crest,
pubmed-meshheading:17179204-Phalloidine,
pubmed-meshheading:17179204-Pseudopodia,
pubmed-meshheading:17179204-Rats,
pubmed-meshheading:17179204-Rats, Sprague-Dawley,
pubmed-meshheading:17179204-Receptors, Eph Family,
pubmed-meshheading:17179204-Solubility
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pubmed:year |
2007
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pubmed:articleTitle |
Ena/VASP proteins mediate repulsion from ephrin ligands.
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pubmed:affiliation |
Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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