Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2006-12-27
pubmed:abstractText
It is thought that gene conversion (GCV) and somatic hypermutation (SHM) of immunoglobulin (Ig) genes occur in two steps: the generation of uracils in DNA by activation-induced cytidine deaminase, followed by their subsequent repair by various DNA repair pathways to generate sequence-diversified products. It is not known how either of the two steps is targeted specifically to Ig loci. Because of the tight link between transcription and SHM, we have investigated the role of endogenous Ig light chain (IgL) transcriptional control elements in GCV/SHM in the chicken B cell line DT40. Promoter substitution experiments led to identification of a strong RNA polymerase II promoter incapable of supporting efficient GCV/SHM. This surprising finding indicates that high levels of transcription are not sufficient for robust GCV/SHM in Ig loci. Deletion of the IgL enhancer in a context in which high-level transcription was not compromised showed that the enhancer is not necessary for GCV/SHM. Our results indicate that cis-acting elements are important for Ig gene diversification, and we propose that targeting specificity is achieved through the combined action of several Ig locus elements that include the promoter.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11007474, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11007475, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11290786, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11528482, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11591226, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11847344, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11882297, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-11904364, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-12065838, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-12202747, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-12214226, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-12692548, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-12692563, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-12932357, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-14684824, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-14768055, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-15252444, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-16186486, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-16342328, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-16365429, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-16705187, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-16785535, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-16818772, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-1698283, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-1913816, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-2111450, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-2155784, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-2258702, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-7596829, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-7617031, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-8168132, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-8206104, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-8223471, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-8574852, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-8881038, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9016873, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9233808, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9293769, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9382923, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9482896, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9485211, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9655488, http://linkedlifedata.com/resource/pubmed/commentcorrection/17178919-9697840
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
203
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2919-28
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:17178919-Actins, pubmed-meshheading:17178919-Animals, pubmed-meshheading:17178919-B-Lymphocytes, pubmed-meshheading:17178919-Bacteriophage T7, pubmed-meshheading:17178919-Cell Line, Tumor, pubmed-meshheading:17178919-Cytidine Deaminase, pubmed-meshheading:17178919-Enhancer Elements, Genetic, pubmed-meshheading:17178919-Gene Conversion, pubmed-meshheading:17178919-Gene Deletion, pubmed-meshheading:17178919-Gene Expression, pubmed-meshheading:17178919-Genes, Immunoglobulin Light Chain, pubmed-meshheading:17178919-Immunoglobulin Heavy Chains, pubmed-meshheading:17178919-Immunoglobulin M, pubmed-meshheading:17178919-Mutation, pubmed-meshheading:17178919-Peptide Elongation Factor 1, pubmed-meshheading:17178919-Promoter Regions, Genetic, pubmed-meshheading:17178919-Receptors, Antigen, B-Cell, pubmed-meshheading:17178919-Somatic Hypermutation, Immunoglobulin, pubmed-meshheading:17178919-Transcription, Genetic, pubmed-meshheading:17178919-Transfection
pubmed:year
2006
pubmed:articleTitle
Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.
pubmed:affiliation
Section of Immunobiology and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
pubmed:publicationType
Journal Article
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