Linked Life Data

17176261

Source:http://linkedlifedata.com/resource/pubmed/id/17176261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-1-31
pubmed:abstractText
Astroglial activation constitutes a dominant response to all types of injuries of the CNS. Despite the ubiquitous nature of this cellular reaction to neural injury, a little is known concerning the signaling mechanisms that initiate it. Recently, we demonstrated that astrocytic hypertrophy and enhanced expression of glial fibrillary acidic protein resulting from toxicant-induced neurodegeneration are linked to activation of the janus kinase (JAK)-signal transducer and activator of transcription-3 (STAT3) pathway. These observations implicate ligands at the gp130 receptor as potential upstream effectors of astrogliosis. Here we used the brain slice preparation to examine potential activators of the JAK-STAT3 pathway. Following incubation of freshly cut striatal slices in phosphate-free oxygenated buffer for up to 75 min, we found that slicing the striatum itself was a sufficient stimulus to initiate a rapid activation of the JAK-STAT3 pathway as assessed with immunoblots of pSTAT3((tyr705)) using phospho-state specific antibodies. The mRNA for the gp130 cytokines, leukemia inhibitory factor, interleukin-6 and oncostatin M or the beta-chemokine, monocyte chemoattractive protein (CCl2) also were up-regulated in the slice. Moreover, we could enhance the activation of STAT3((tyr705)) by adding exogenous cytokines to the slice and we could inhibit phosphorylation of STAT3((tyr705)) by addition of tyrosine kinase inhibitors (Lav A and AG490) or neutralizing antibodies directed against leukemia inhibitory factor or oncostatin M. These data suggest that STAT3 activation is an early event in slice-induced glial activation and establishes the brain slice preparation method as a reliable model to examine the signaling mechanisms that underlie glial activation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
720-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17176261-Animals, pubmed-meshheading:17176261-Antibodies, pubmed-meshheading:17176261-Astrocytes, pubmed-meshheading:17176261-Brain, pubmed-meshheading:17176261-Brain Injuries, pubmed-meshheading:17176261-Chemokines, pubmed-meshheading:17176261-Cytokines, pubmed-meshheading:17176261-Enzyme Activation, pubmed-meshheading:17176261-Enzyme Inhibitors, pubmed-meshheading:17176261-Female, pubmed-meshheading:17176261-Glial Fibrillary Acidic Protein, pubmed-meshheading:17176261-Gliosis, pubmed-meshheading:17176261-Janus Kinase 1, pubmed-meshheading:17176261-Mice, pubmed-meshheading:17176261-Mice, Inbred C57BL, pubmed-meshheading:17176261-Models, Biological, pubmed-meshheading:17176261-Organ Culture Techniques, pubmed-meshheading:17176261-STAT3 Transcription Factor, pubmed-meshheading:17176261-Signal Transduction, pubmed-meshheading:17176261-Up-Regulation
pubmed:year
2007
pubmed:articleTitle
Recapitulation of cell signaling events associated with astrogliosis using the brain slice preparation.
pubmed:affiliation
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health (CDC-NIOSH), Morgantown, WV 26505, USA.
pubmed:publicationType
Journal Article