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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
29
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pubmed:dateCreated |
1991-11-14
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated the gene encoding the neuronal intermediate filament protein alpha-internexin using low stringency hybridization conditions and an NF-M (neurofilament middle molecular weight subunit) cDNA probe. This gene consists of three exons and two introns. The sequence data and the exon-intron organization of the gene establish its classification as a type IV intermediate filament gene. Transient transfection experiments showed that up to 5 kilobases of the alpha-internexin promoter region transcribed equally well in both the alpha-internexin-expressing and -nonexpressing cell lines. The results also demonstrated that the region from -77 to +73 relative to the transcription start site was sufficient for accurate basal transcription, but inclusion of the -254 to -78 region was required for efficient transcription. Sequence analysis shows that the -254 to -78 region contains several potential positive regulatory elements.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19459-68
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1717465-Amino Acid Sequence,
pubmed-meshheading:1717465-Animals,
pubmed-meshheading:1717465-Base Sequence,
pubmed-meshheading:1717465-Blotting, Northern,
pubmed-meshheading:1717465-Carrier Proteins,
pubmed-meshheading:1717465-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1717465-DNA,
pubmed-meshheading:1717465-Exons,
pubmed-meshheading:1717465-Genes,
pubmed-meshheading:1717465-Intermediate Filament Proteins,
pubmed-meshheading:1717465-Liver,
pubmed-meshheading:1717465-Molecular Sequence Data,
pubmed-meshheading:1717465-Molecular Weight,
pubmed-meshheading:1717465-Plasmids,
pubmed-meshheading:1717465-Protein Biosynthesis,
pubmed-meshheading:1717465-RNA,
pubmed-meshheading:1717465-Rats,
pubmed-meshheading:1717465-Restriction Mapping,
pubmed-meshheading:1717465-Transcription, Genetic,
pubmed-meshheading:1717465-Transfection
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pubmed:year |
1991
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pubmed:articleTitle |
Structure of the gene for the neuronal intermediate filament protein alpha-internexin and functional analysis of its promoter.
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pubmed:affiliation |
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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