Linked Life Data

17172832

Source:http://linkedlifedata.com/resource/pubmed/id/17172832

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2006-12-25
pubmed:abstractText
Expression of the viral protein R, Vpr, of HIV-1 affects many biological events in host cells including cell cycle progression, and modulates HIV-1 gene transcription. Earlier studies implicating the cellular protein p21(WAF1) (p21) in regulation of HIV-1 transcription, led us to investigate the functional and physical interaction of Vpr and p21. Our results show that Vpr modestly activated HIV-LTR in cells lacking p21 gene. Here, we describe the mechanisms by which p21 and Vpr leading to stimulation of HIV-1 transcription. Data from the protein-protein interaction experiments revealed the ability of Vpr, p21 and p300 to form a complex. Further, we show that, Vpr interacts with the N- and the C-terminal domains of p21. Furthermore, in cells expressing Vpr, p21 localizes to both the cytoplasm and the nucleus. Interestingly, expression of Vpr alleviates p21-mediated inhibition of cell departure from G1 phase. Expression of a mutant Vpr, with arginine 73 altered to serine, did not affect the ability of p21 to cause cells arrest or its sub-cellular localization. These observations reveal a new cellular partner for Vpr, and provide a new therapeutic avenue for controlling HIV-1 expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1551-4005
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2626-38
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:17172832-Binding Sites, pubmed-meshheading:17172832-Cell Cycle, pubmed-meshheading:17172832-Cell Line, Tumor, pubmed-meshheading:17172832-Cell Nucleus, pubmed-meshheading:17172832-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:17172832-Cytoplasm, pubmed-meshheading:17172832-E1A-Associated p300 Protein, pubmed-meshheading:17172832-Gene Expression Regulation, Viral, pubmed-meshheading:17172832-Gene Products, vpr, pubmed-meshheading:17172832-HCT116 Cells, pubmed-meshheading:17172832-HIV Long Terminal Repeat, pubmed-meshheading:17172832-HIV-1, pubmed-meshheading:17172832-Humans, pubmed-meshheading:17172832-Microscopy, Fluorescence, pubmed-meshheading:17172832-Models, Biological, pubmed-meshheading:17172832-Transfection, pubmed-meshheading:17172832-vpr Gene Products, Human Immunodeficiency Virus
pubmed:year
2006
pubmed:articleTitle
The role of Vpr in the regulation of HIV-1 gene expression.
pubmed:affiliation
Department of Neuroscience & Center for Neurovirology, Temple University School of Medicine, Temple University, Philadelphia, Pennsylvania 19122, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural