Linked Life Data

17172047

Source:http://linkedlifedata.com/resource/pubmed/id/17172047

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-12-18
pubmed:databankReference
pubmed:abstractText
OsPT6:1, a phosphate transporter encoding gene from the leaf samples of Oryza sativa, was identified through PCR with specifically designed primers. The phylogenetic analysis and the conserved amino acid residue site detection suggested OsPT6:1 a possible high-affinity phosphate transporter encoding gene. In situ hybridization and RT-PCR demonstrated the expression of OsPT6:1 in both roots and leaves. The peak expression signal was observed in mesophyll cells under low phosphorus (P) induction. A homologous recombination study indicated that OsPT6:1 can enhance the Pi uptake efficiency of Pichia pastoris. At the meantime, the introduction of OsPT6:1 was able to complement the Pi uptake function of yeast cells with high-affinity phosphate transporters deficient. Those results substantiated our contention that OsPT6:1 encoded a high-affinity phosphate transporter of Oryza sativa.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1006-9305
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-13
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Cloning, expression and function of phosphate transporter encoded gene in Oryza sativa L.
pubmed:affiliation
Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai 200433, China. fming@fudan.edu.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't