17172012

Source:http://linkedlifedata.com/resource/pubmed/id/17172012

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0080194, umls-concept:C0204727, umls-concept:C0205409, umls-concept:C0441655, umls-concept:C0600148, umls-concept:C1519068
pubmed:issue	5
pubmed:dateCreated	2006-12-18
pubmed:abstractText	One facultative alkaliphilic actinomycete strain YIM 90022 was isolated from hypersaline alkaline soil in Qinghai province, China. An almost-complete 16S rRNA gene sequence (1500 bp) for strain YIM 90022 was obtained. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 90022 was closely related to four members of the genus Nocardiopsis with 16S rRNA gene sequence similarity values of 98.8% (N. exhalans DSM 44407T), 98.5% (N. prasina DSM 43845T), 98.4% (N. metallicus DSM 44598T) and 97.8% (N. listeri DSM 40297T), but represented a distinct phylogenetic lineage. Repetitive element sequence-based PCR (rep-PCR) genomic fingerprinting was evaluated on strain YIM 90022 and its closest relatives to investigate their genetic relatedness. The analysis of the rep-PCR genomic fingerprints showed that strain YIM 90022 was distinguishable from its closest relatives. The polyphasic taxonomic data presented in this study, including its morphology, physiological and biochemical characteristics, chemotaxonomy, 16S rRNA gene sequence-based phylogenetic analysis and rep-PCR genomic fingerprinting, supported the view that strain YIM 90022 represented a potential new species of the genus Nocardiopsis. The fermentation broth of strain YIM 90022 strongly inhibited growth of cell series of gastric cancer, lung cancer, mammary cancer, melanoma cancer, renal cancer and uterus cancer. Strain YIM 90022 grew well on most tested media, producing exuberant vegetative hyphae and aerial hyphae. The vegetative hyphae are long and fragmented. Light yellow to deep brown diffusible pigments were produced on ISP 2, ISP 3 and ISP 6. Growth of the strain occurred in the pH range 6.0-12.0, with optimal pH8.5. The NaCl tolerate range was 0-15% (W/V). Cell walls contain meso-diaminopimelic acid and have no diagnostic sugars. Polar lipids are phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine. Major menaquinones are MK-10 (H4, H6). The DNA G + C content is 71.5 mol %.
pubmed:language	chi
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/21610860R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	0001-6209
pubmed:author	pubmed-author:ChenYi-GuangYG, pubmed-author:CuiXiao-LongXL, pubmed-author:JiangCheng-LinCL, pubmed-author:LiWen-JunWJ, pubmed-author:XuLi-HuaLH
pubmed:issnType	Print
pubmed:volume	46
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	696-701
pubmed:meshHeading	pubmed-meshheading:17172012-Actinobacteria, pubmed-meshheading:17172012-Antibiotics, Antineoplastic, pubmed-meshheading:17172012-Nucleotide Mapping, pubmed-meshheading:17172012-Phylogeny, pubmed-meshheading:17172012-Polymerase Chain Reaction, pubmed-meshheading:17172012-RNA, Ribosomal, 16S
pubmed:year	2006
pubmed:articleTitle	[Isolation and phylogenetic analysis of one actinomycete strain YIM 90022 exhibiting anticancer activity].
pubmed:affiliation	Key Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology Yunnan University, Kunming 650091, China. mchenjsu@tom.com
pubmed:publicationType	Journal Article, English Abstract, Research Support, Non-U.S. Gov't