Source:http://linkedlifedata.com/resource/pubmed/id/17171761
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2006-12-27
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| pubmed:abstractText |
The considerable potential of Cre recombinase as a tool for in vivo fate-mapping studies depends on the availability of reliable reporter mice. By targeting a tandem-dimer red fluorescent protein (tdRFP) with advanced spectral and biological properties into the ubiquitously expressed ROSA26 locus of C57BL/6-ES cells, we have generated a novel inbred Cre-reporter mouse with several unique characteristics. We directly demonstrate the usefulness of our reporter strain in inter-crosses with a "universal Cre-deleter" strain and with mice expressing Cre recombinase in a T lineage-specific manner. Cytofluorometric and histological analyses illustrate: (i) non-toxicity and extraordinary brightness of the fluorescent reporter, allowing quantitative detection and purification of labeled cells with highest accuracy, (ii) reliable Cre-mediated activation of tdRFP from an antisense orientation relative to ROSA26 transcription, effectively excluding "leaky" reporter expression, (iii) absence of gene expression variegation effects, (iv) quantitative detection of tdRFP-expressing cells even in paraformaldehyde-fixed tissue sections, and (v) full compatibility with GFP/YFP-based fluorescent markers in multicolor experiments. Taken together, the data show that our C57BL/6-inbred reporter mice are ideally suited for sophisticated lineage-tracing experiments requiring sensitive and quantitative detection/purification of live Cre-expressing cells and their progeny.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Lox protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Lysine 6-Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/red fluorescent protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0014-2980
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
37
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
43-53
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| pubmed:meshHeading |
pubmed-meshheading:17171761-Animals,
pubmed-meshheading:17171761-Cell Line,
pubmed-meshheading:17171761-Cell Lineage,
pubmed-meshheading:17171761-Crosses, Genetic,
pubmed-meshheading:17171761-Extracellular Matrix Proteins,
pubmed-meshheading:17171761-Female,
pubmed-meshheading:17171761-Gene Expression Regulation,
pubmed-meshheading:17171761-Genes, Reporter,
pubmed-meshheading:17171761-Integrases,
pubmed-meshheading:17171761-Luminescent Proteins,
pubmed-meshheading:17171761-Male,
pubmed-meshheading:17171761-Mice,
pubmed-meshheading:17171761-Mice, Inbred BALB C,
pubmed-meshheading:17171761-Mice, Inbred C57BL,
pubmed-meshheading:17171761-Mice, Inbred DBA,
pubmed-meshheading:17171761-Mice, Inbred Strains,
pubmed-meshheading:17171761-Mice, Mutant Strains,
pubmed-meshheading:17171761-Mice, Transgenic,
pubmed-meshheading:17171761-Protein-Lysine 6-Oxidase
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| pubmed:year |
2007
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| pubmed:articleTitle |
Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.
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| pubmed:affiliation |
Institute of Immunology, University Clinics Ulm, Ulm, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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