17170460

Source:http://linkedlifedata.com/resource/pubmed/id/17170460

Download in:

Switch to

Custom View

Named Graph Language Inference

Statements in which the resource exists as a subject.
Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0006104, umls-concept:C0019381, umls-concept:C0026769, umls-concept:C0030705, umls-concept:C0035366, umls-concept:C0456644, umls-concept:C0600466, umls-concept:C1422036, umls-concept:C2243049
pubmed:issue	Pt 1
pubmed:dateCreated	2006-12-15
pubmed:abstractText	Multiple sclerosis (MS)-associated retrovirus (MSRV)/HERV-W (human endogenous retrovirus W) and Human herpesvirus 6 (HHV-6) are the two most studied (and discussed) viruses as environmental co-factors that trigger MS immunopathological phenomena. Autopsied brain tissues from MS patients and controls and peripheral blood mononuclear cells (PBMCs) were analysed. Quantitative RT-PCR and PCR with primers specific for MSRV/HERV-W env and pol and HHV-6 U94/rep and DNA-pol were used to determine virus copy numbers. Brain sections were immunostained with HERV-W env-specific monoclonal antibody to detect the viral protein. All brains expressed MSRV/HERV-W env and pol genes. Phylogenetic analysis indicated that cerebral MSRV/HERV-W-related env sequences, plasmatic MSRV, HERV-W and ERVWE1 (syncytin) are related closely. Accumulation of MSRV/HERV-W-specific RNAs was significantly greater in MS brains than in controls (P=0.014 vs healthy controls; P=0.006 vs pathological controls). By immunohistochemistry, no HERV-W env protein was detected in control brains, whereas it was upregulated within MS plaques and correlated with the extent of active demyelination and inflammation. No HHV-6-specific RNAs were detected in brains of MS patients; one healthy control had latent HHV-6 and one pathological control had replicating HHV-6. At the PBMC level, all MS patients expressed MSRV/HERV-W env at higher copy numbers than did controls (P=0.00003). Similar HHV-6 presence was found in MS patients and healthy individuals; only one MS patient had replicating HHV-6. This report, the first to study both MSRV/HERV-W and HHV-6, indicates that MSRV/HERV-W is expressed actively in human brain and activated strongly in MS patients, whilst there are no significant differences between these MS patients and controls for HHV-6 presence/replication at the brain or PBMC level.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0077340
pubmed:citationSubset	IM
pubmed:status	MEDLINE
pubmed:month	Jan
pubmed:issn	0022-1317
pubmed:author	pubmed-author:ArruGianninaG, pubmed-author:AstoneVitoV, pubmed-author:BonettiBrunoB, pubmed-author:DoleiAntoninaA, pubmed-author:LovatoLauraL, pubmed-author:MameliGiuseppeG, pubmed-author:MarconiSilviaS, pubmed-author:SerraCaterinaC, pubmed-author:SotgiuStefanoS
pubmed:issnType	Print
pubmed:volume	88
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	264-74
pubmed:meshHeading	pubmed-meshheading:17170460-Brain, pubmed-meshheading:17170460-Endogenous Retroviruses, pubmed-meshheading:17170460-Herpesvirus 6, Human, pubmed-meshheading:17170460-Humans, pubmed-meshheading:17170460-Leukocytes, Mononuclear, pubmed-meshheading:17170460-Multiple Sclerosis
pubmed:year	2007
pubmed:articleTitle	Brains and peripheral blood mononuclear cells of multiple sclerosis (MS) patients hyperexpress MS-associated retrovirus/HERV-W endogenous retrovirus, but not Human herpesvirus 6.
pubmed:affiliation	Section of Microbiology, Department of Biomedical Sciences, Center of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Sassari, Italy.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't