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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-3-19
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pubmed:abstractText |
Fibroblast growth factor (FGF)-2 regulates chondrocyte proliferation in the growth plate. Heparan sulfate (HS) proteoglycans bind FGF-2. Perlecan, a heparan sulfate proteoglycan (HSPG) in the developing growth plate, however, contains both HS and chondroitin sulfate (CS) chains. The binding of FGF-2 to perlecan isolated from the growth plate was evaluated using cationic filtration (CAF) and immunoprecipitation (IP) assays. FGF-2 bound to perlecan in both the CAF and IP assays primarily via the HS chains on perlecan. A maximum of 123 molecules of FGF-2 was calculated to bind per molecule of perlecan. When digested with chondroitinase ABC to remove its CS chains, perlecan augmented binding of FGF-2 to the FGFR-1 and FGFR-3 receptors and also increased FGF-2 stimulation of [(3)H]-thymidine incorporation in BaF3 cells expressing these FGF receptors. These data show that growth plate perlecan binds to FGF-2 by its HS chains but can only deliver FGF-2 to FGF receptors when its CS chains are removed.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0945-053X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-84
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pubmed:dateRevised |
2007-12-10
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pubmed:meshHeading |
pubmed-meshheading:17169545-Animals,
pubmed-meshheading:17169545-Cattle,
pubmed-meshheading:17169545-Chondroitin Sulfates,
pubmed-meshheading:17169545-Chondroitinases and Chondroitin Lyases,
pubmed-meshheading:17169545-Fibroblast Growth Factor 2,
pubmed-meshheading:17169545-Growth Plate,
pubmed-meshheading:17169545-Heparan Sulfate Proteoglycans,
pubmed-meshheading:17169545-Heparitin Sulfate,
pubmed-meshheading:17169545-Humans,
pubmed-meshheading:17169545-Polysaccharide-Lyases,
pubmed-meshheading:17169545-Receptors, Fibroblast Growth Factor
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pubmed:year |
2007
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pubmed:articleTitle |
Heparan and chondroitin sulfate on growth plate perlecan mediate binding and delivery of FGF-2 to FGF receptors.
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pubmed:affiliation |
Department of Molecular Medicine, College of Medicine, University of South Florida, 12901 Bruce B Downs Blvd., MDC Box 7 Tampa, FL 33612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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