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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-12-15
pubmed:abstractText
BMS-299897 is a gamma-secretase inhibitor that has the potential for treatment of Alzheimer's disease. The metabolism of [(14)C]BMS-299897 was investigated in human liver microsomes, in rat, dog, monkey and human hepatocytes and in bile duct cannulated rats. Seven metabolites (M1-M7) were identified from in vitro and in vivo studies. LC-MS/MS analysis showed that M1 and M2 were regioisomeric acylglucuronide conjugates of BMS-299897. Metabolites M3, M4 and M6 were identified as monohydroxylated metabolites of BMS-299897 and M5 was identified as the dehydrogenated product of monooxygenated BMS-299897. In vivo, 52% of the radioactive dose was excreted in bile within 0-6 h from bile duct cannulated rats following a single oral dose of 15 mg/kg of [(14)C]BMS-299897. Glucuronide conjugates, M1 and M2 accounted for 80% of the total radioactivity in rat bile. In addition to M1 and M2, M7 was observed in rat bile which was identified as a glucuronide conjugate of an oxidative metabolite M5. For structure elucidation and pharmacological activity testing of the metabolites, ten microbial cultures were screened for their ability to metabolize BMS-299897 to form these metabolites. Among them, the fungus Cunninghamella elegans produced two major oxidative metabolites M3 and M4 that had the same HPLC retention time and mass spectral properties as those found in in vitro incubations. NMR analysis indicated that M3 and M4 were stereoisomers, with the hydroxyl group on the benzylic position. However, M3 and M4 were unstable and converted to their corresponding lactones readily. Based on x-ray analysis of the synthetically prepared lactone of M3, the stereochemistry of benzylic hydroxyl group was assigned as the R configuration. Both the hydroxy metabolites (M3 and M4) and the lactone of M3 showed gamma-secretase inhibition with IC(50) values similar to that of the parent compound. This study demonstrates the usefulness of microbial systems as bioreactors to generate metabolites of BMS-299897 in large quantities for structure elucidation and activity testing. This study also demonstrates the biotransformation profile of BMS-299897 is qualitatively similar across the species including rat, dog, monkey and human which provides a basis to support rat, dog and monkey as preclinical models for toxicological testing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1389-2002
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
883-96
pubmed:meshHeading
pubmed-meshheading:17168689-Amyloid Precursor Protein Secretases, pubmed-meshheading:17168689-Animals, pubmed-meshheading:17168689-Bile, pubmed-meshheading:17168689-Bioreactors, pubmed-meshheading:17168689-Biotransformation, pubmed-meshheading:17168689-Butyric Acids, pubmed-meshheading:17168689-Carbon Radioisotopes, pubmed-meshheading:17168689-Cell Line, Tumor, pubmed-meshheading:17168689-Cells, Cultured, pubmed-meshheading:17168689-Cunninghamella, pubmed-meshheading:17168689-Dogs, pubmed-meshheading:17168689-Drug Evaluation, Preclinical, pubmed-meshheading:17168689-Enzyme Inhibitors, pubmed-meshheading:17168689-Glucuronides, pubmed-meshheading:17168689-Hepatocytes, pubmed-meshheading:17168689-Humans, pubmed-meshheading:17168689-Hydrocarbons, Halogenated, pubmed-meshheading:17168689-Macaca fascicularis, pubmed-meshheading:17168689-Male, pubmed-meshheading:17168689-Microsomes, Liver, pubmed-meshheading:17168689-Rats, pubmed-meshheading:17168689-Rats, Sprague-Dawley
pubmed:year
2006
pubmed:articleTitle
In vitro and in vivo metabolism of a gamma-secretase inhibitor BMS-299897 and generation of active metabolites in milligram quantities with a microbial bioreactor.
pubmed:affiliation
Pharmaceutical Candidate Optimization Department, Bristol Myers Squibb Pharmaceutical Co., Route 206 and Province Line Road, Princeton, NJ 08543, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't