Source:http://linkedlifedata.com/resource/pubmed/id/17166228
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2007-2-22
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pubmed:abstractText |
To study the possibility of utilizing genetically engineered Leifsonia xyli subsp. cynodontis (Lxc) as an endophytic bacterium in rice, we constructed an Escherichia coli-Lxc shuttle vector, pLGUS, containing a beta-glucuronidase reporter gene, which was stable both in vitro and in vivo. Lxc grows and expresses the beta-glucuronidase reporter gene in all parts of rice, except for seed. A 2-year field study using three rice varieties from China showed that Lxc inoculation did not have a negative effect on the growth and yield of any of these varieties. Therefore, Lxc has the potential to be used as a benign endophyte for the expression of foreign genes in rice.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
176-83
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pubmed:meshHeading |
pubmed-meshheading:17166228-Actinomycetales,
pubmed-meshheading:17166228-Escherichia coli,
pubmed-meshheading:17166228-Genes, Reporter,
pubmed-meshheading:17166228-Genetic Vectors,
pubmed-meshheading:17166228-Glucuronidase,
pubmed-meshheading:17166228-Oryza sativa,
pubmed-meshheading:17166228-Plasmids
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pubmed:year |
2007
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pubmed:articleTitle |
Construction of a stable expression vector for Leifsonia xyli subsp. cynodontis and its application in studying the effect of the bacterium as an endophytic bacterium in rice.
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pubmed:affiliation |
Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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