Linked Life Data

17165548

Source:http://linkedlifedata.com/resource/pubmed/id/17165548

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-12-14
pubmed:abstractText
A simple, sensitive, and specific gas chromatographic (GC) method was developed to determine the main bioactive sesquiterpene lactones, trilobolide-6-O-isobutyrates A and B (TBO-A and TBO-B), in Wedelia trilobata, a useful folk herb. A commercially available HP-5 capillary column (30 m x 0.25 mm i.d. x 0.25 microm) was utilized for the direct determination of TBO-A and TBO-B in W. trilobata. Calibration curves were obtained by spiking authentic compounds and the internal standard (ferulic acid) into W. trilobata samples before extraction. Extraction was carried out by refluxing the dried herb (0.5 g) for 1 h in methanol (25 mL). All calibration curves showed good linear regressions (r2 > 0.992) within test ranges. The assay was reproducible and accurate with the overall intraday and interday relative standard deviations and accuracies of less than 10% and more than 90%, respectively. The developed GC method was successfully utilized to analyze the TBO-A and TBO-B in aerial parts and flowers of W. trilobata, indicating that it was suitable for the quality control of this commonly used herb and related traditional Chinese medicines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1000-8713
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-502
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Simultaneous determination of trilobolide-6-O-isobutyrates A and B in Wedelia trilobata by gas chromatography.
pubmed:affiliation
Department of Food Science and Technology, College of Science and Engineering, Jinan University, Guangzhou 510632, China. thxs1957@yahoo.com.cn
pubmed:publicationType
Journal Article