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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
1991-10-24
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pubmed:abstractText |
Immunologic measurements of the serum concentration of prostate-specific antigen (PSA), an abundant prostatic-secreted serine proteinase, are frequently used to monitor patients with prostate cancer, though it has not been ascertained whether this immunoreactivity represents a PSA zymogen, the active proteinase, or PSA complexed to extracellular proteinase inhibitors. To characterize the PSA immunoreactivity in serum, we used monoclonal antibodies produced against PSA and a polyclonal rabbit IgG against alpha 1-antichymotrypsin in the design of three noncompetitive PSA assays: assay T, which detected PSA both when present as the active proteinase and when complexed to alpha 1-antichymotrypsin; assay F, which recognized the active proteinase but most poorly detected PSA complexed to alpha 1-antichymotrypsin; and assay C, which was specific for PSA complexed to alpha 1-antichymotrypsin. We used the three assays to measure PSA immunoreactivity in 64 patients' sera and in the effluent after gel chromatography of sera from four patients. This identified an 80- to 90-kDa complex between PSA and alpha 1-antichymotrypsin as the predominant fraction of the PSA immunoreactivity in blood plasma; an immunoreactive 25- to 40-kDa compound was the minor fraction.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Prostate-Specific Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/alpha 1-Antichymotrypsin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1618-25
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pubmed:dateRevised |
2010-12-15
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pubmed:meshHeading |
pubmed-meshheading:1716536-Animals,
pubmed-meshheading:1716536-Antibodies, Monoclonal,
pubmed-meshheading:1716536-Antigens, Neoplasm,
pubmed-meshheading:1716536-Chromatography, Gel,
pubmed-meshheading:1716536-Electrophoresis, Agar Gel,
pubmed-meshheading:1716536-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1716536-Epitopes,
pubmed-meshheading:1716536-Female,
pubmed-meshheading:1716536-Humans,
pubmed-meshheading:1716536-Male,
pubmed-meshheading:1716536-Mice,
pubmed-meshheading:1716536-Mice, Inbred BALB C,
pubmed-meshheading:1716536-Prostate-Specific Antigen,
pubmed-meshheading:1716536-Tumor Markers, Biological,
pubmed-meshheading:1716536-alpha 1-Antichymotrypsin
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pubmed:year |
1991
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pubmed:articleTitle |
Prostate-specific antigen in serum occurs predominantly in complex with alpha 1-antichymotrypsin.
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pubmed:affiliation |
Department of Clinical Chemistry, University of Lund, Malmö General Hospital, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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