|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
1976-1-16
|
|
pubmed:abstractText |
The ffect of N-ethylmaleimide and iodoacetamide on the glucocorticoid receptor activity extracted from the cytosol of either mouse of hamster cells has been investigated. Treatment of mouse or hamster cytosol with N-ethylmaleimide or iodoacetamide rapidly inactivates the [3H]glucocorticoid hormone binding activity of either cytosol. Prebinding the glucocorticoid hormone, dexamethasone, to the cytosol receptor blocks the rapid inactivation of the receptor by N-ethylmaleimide. Treatment of the prebound hormone-receptor complex with iodoacetamide prevents the subsequent binding of the hormone-receptor complex to DNA without causing a dissociation of the complex. Although the conclusions may be limited by the lack of purity of the receptor, the results suggest that a sulfhydryl group is involved in the binding of glucocorticoid hormones to the receptor protein. In addition, the results suggest that iodoacetamide is inactivating a separate chemical site which is necessary for the binding of the hormone-receptor complex to DNA.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-1055360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-163248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-163765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-164467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4109409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4152030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4326936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4356254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4361099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4370191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4372597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4373173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4563437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-47326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4752279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-4896248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-5012467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/171651-5723464
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Dithiothreitol,
http://linkedlifedata.com/resource/pubmed/chemical/Ethylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids,
http://linkedlifedata.com/resource/pubmed/chemical/Iodoacetamide,
http://linkedlifedata.com/resource/pubmed/chemical/Iodoacetates,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Manganese,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
72
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3060-4
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:171651-Binding Sites,
pubmed-meshheading:171651-Calcium,
pubmed-meshheading:171651-Cell Line,
pubmed-meshheading:171651-Cytosol,
pubmed-meshheading:171651-Dexamethasone,
pubmed-meshheading:171651-Dithiothreitol,
pubmed-meshheading:171651-Ethylmaleimide,
pubmed-meshheading:171651-Glucocorticoids,
pubmed-meshheading:171651-Iodoacetamide,
pubmed-meshheading:171651-Iodoacetates,
pubmed-meshheading:171651-Kinetics,
pubmed-meshheading:171651-Magnesium,
pubmed-meshheading:171651-Manganese,
pubmed-meshheading:171651-Protein Binding,
pubmed-meshheading:171651-Receptors, Cell Surface,
pubmed-meshheading:171651-Temperature
|
|
pubmed:year |
1975
|
|
pubmed:articleTitle |
Effect of chemical inactivating agents on glucocorticoid receptor proteins in mouse and hamster cells.
|
|
pubmed:publicationType |
Journal Article
|
