Source:http://linkedlifedata.com/resource/pubmed/id/17164408
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2007-3-19
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pubmed:abstractText |
We evaluated the IGF1 system in cholangiocytes of primay biliary cirrhosis (PBC) patients and investigated the relationships with apoptosis. Biopsies of PBC patients (n=32) and normal subjects (n=5) were investigated by immunohistochemistry for expression in cholangiocytes of IGF1, IGF1-R, pAKT, terminal deoxynucleotide transferase end labeling (TUNEL), Bax (proapoptotic protein), and Bcl2 (antiapoptotic protein). Whereas normal cholangiocytes were almost negative, cholangiocytes of PBC patients showed strong IHC staining for IGF1, IGF1-R, and pAKT, which increases from stage I to stage IV, where >70% of cholangiocytes were positive. Bax/Bcl2 ratio reached the highest value (4.6) in PBC stage III when apoptosis is maximal (24% TUNEL positivity), whereas it declines in stage IV (1.4) when only 7.8% cholangiocytes were TUNEL positive. In PBC stages III and IV, expression of IGF1, IGF1-R, and pAKT in cholangiocytes was directly correlated with the antiapoptotic Bcl2 and inversely correlated with proapoptotic Bax, Bax/Bcl2 ratio, and TUNEL positivity. In conclusion, cholangiocytes of PBC patients showed a marked increase in IGF1, IGF1-R, and pAKT expression involving most cholangiocytes surviving in the terminal ductopenic stage. This was associated and correlated with a balance of pro- and antiapoptotic proteins favoring survival rather than apoptosis, suggesting a major role of IGF1 system in promoting cholangiocyte survival.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1554
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pubmed:author |
pubmed-author:AlvaroDomenicoD,
pubmed-author:AngelicoMarioM,
pubmed-author:AttiliAdolfo FAF,
pubmed-author:CarpinoGuidoG,
pubmed-author:De SantisAdrianoA,
pubmed-author:FloreaniAnna RosaAR,
pubmed-author:FranchittoAntonioA,
pubmed-author:GaudioEugenioE,
pubmed-author:GuidoMariaM,
pubmed-author:MancinoMaria GraziaMG,
pubmed-author:OnoriPaoloP
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
327-34
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17164408-Apoptosis,
pubmed-meshheading:17164408-Bile Ducts,
pubmed-meshheading:17164408-Cell Survival,
pubmed-meshheading:17164408-Disease Progression,
pubmed-meshheading:17164408-Female,
pubmed-meshheading:17164408-Humans,
pubmed-meshheading:17164408-Immunohistochemistry,
pubmed-meshheading:17164408-In Situ Nick-End Labeling,
pubmed-meshheading:17164408-Insulin-Like Growth Factor I,
pubmed-meshheading:17164408-Liver Cirrhosis, Biliary,
pubmed-meshheading:17164408-Middle Aged,
pubmed-meshheading:17164408-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:17164408-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:17164408-bcl-2-Associated X Protein
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pubmed:year |
2007
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pubmed:articleTitle |
Activation of the IGF1 system characterizes cholangiocyte survival during progression of primary biliary cirrhosis.
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pubmed:affiliation |
Department of Experimental Medicine, University of L'Aquila, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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