Source:http://linkedlifedata.com/resource/pubmed/id/17159239
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2006-12-12
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| pubmed:abstractText |
Radiation therapy has been used in the treatment of a wide variety of cancers for nearly a century and is one of the most effective ways to treat cancer. Low-dose ionizing radiation (IR) can interfere with cell division of cancer and normal cells by introducing oxidative stress and injury to DNA. The differences in the response to IR-induced DNA damage and increased reactive oxygen species between normal human fibroblasts (NHFs) and cancerous SHSY-5Y cells were considered. H2AX staining and comet assays revealed that NHF cells responded by initiating a DNA repair sequence whereas SHSY-5Y cells did not. In addition, NHF cells appeared to quench the oxidative stress induced by IR, and after 24 h no DNA damage was present. SHSY-5Y cells, however, did not repair their DNA, did not quench the oxidative stress, and showed characteristic signs that they were beginning to undergo apoptosis. These results indicate that there is a differential response between this cancerous and normal cell line in their ability to respond to low-dose IR, and these differences need to be exploited in order to treat cancer effectively. Further study is needed in order to elucidate the mechanism by which SHSY-5Y cells undergo apoptosis following radiation and why these normal cells are better equipped to deal with IR-induced double-strand breaks and oxidative stress.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/H2AFX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Trypan Blue
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0273-2289
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
135
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
159-78
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| pubmed:meshHeading |
pubmed-meshheading:17159239-Adenosine Triphosphate,
pubmed-meshheading:17159239-Apoptosis,
pubmed-meshheading:17159239-Caspase 3,
pubmed-meshheading:17159239-Cell Nucleus,
pubmed-meshheading:17159239-Comet Assay,
pubmed-meshheading:17159239-DNA Damage,
pubmed-meshheading:17159239-DNA Repair,
pubmed-meshheading:17159239-Fibroblasts,
pubmed-meshheading:17159239-Histones,
pubmed-meshheading:17159239-Humans,
pubmed-meshheading:17159239-Membrane Potential, Mitochondrial,
pubmed-meshheading:17159239-Neuroblastoma,
pubmed-meshheading:17159239-Proteasome Endopeptidase Complex,
pubmed-meshheading:17159239-Radiation, Ionizing,
pubmed-meshheading:17159239-Reactive Oxygen Species,
pubmed-meshheading:17159239-Staining and Labeling,
pubmed-meshheading:17159239-Trypan Blue,
pubmed-meshheading:17159239-Tumor Cells, Cultured
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| pubmed:year |
2006
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| pubmed:articleTitle |
Differential response induced by exposure to low-dose ionizing radiation in SHSY-5Y and normal human fibroblast cells.
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| pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada N9B 3P4.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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