Linked Life Data

17159215

Source:http://linkedlifedata.com/resource/pubmed/id/17159215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 12
pubmed:dateCreated
2006-12-12
pubmed:abstractText
The gene slr0388 was previously annotated to encode a hypothetical protein in Synechocystis sp. strain PCC 6803. When a positively phototactic strain of this cyanobacterium was insertionally inactivated at slr0388, the mutants were not transformable, and appeared to aggregate as a result of increased bundling of type IV pili. Also, these mutants were rendered non-phototactic compared to the wild-type. Quantitative real-time PCR revealed a 3.5-fold increase in pilA1 transcript levels in the mutant over wild-type cells, while there were no changes in the level of pilT1 and comA transcripts. Supernatant from mutant liquid culture contained more PilA1 protein, confirmed by mass spectrometric analysis, compared to the wild-type cells, which corresponded to the increase in pilA1 transcripts. The increase in PilA1 subunits may contribute to the bundling morphology of pili that was observed, which in turn may act to retard DNA uptake by hindering the retraction of pili. This gene is therefore proposed to be designated comF, as it possesses a phosphoribosyltransferase domain, a distinguishing feature of other ComF proteins of naturally transformable heterotrophic bacteria. This report is the second of a competence-related gene from Synechocystis sp. strain PCC 6803, the product of which does not show homology to other well-studied type IV pili proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3623-31
pubmed:meshHeading
pubmed-meshheading:17159215-Amino Acid Sequence, pubmed-meshheading:17159215-Bacterial Proteins, pubmed-meshheading:17159215-DNA Transposable Elements, pubmed-meshheading:17159215-Fimbriae, Bacterial, pubmed-meshheading:17159215-Fimbriae Proteins, pubmed-meshheading:17159215-Light, pubmed-meshheading:17159215-Microscopy, Electron, Scanning, pubmed-meshheading:17159215-Microscopy, Electron, Transmission, pubmed-meshheading:17159215-Molecular Sequence Data, pubmed-meshheading:17159215-Movement, pubmed-meshheading:17159215-Mutagenesis, Insertional, pubmed-meshheading:17159215-RNA, Bacterial, pubmed-meshheading:17159215-RNA, Messenger, pubmed-meshheading:17159215-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17159215-Sequence Alignment, pubmed-meshheading:17159215-Synechocystis, pubmed-meshheading:17159215-Transformation, Bacterial
pubmed:year
2006
pubmed:articleTitle
The competence gene, comF, from Synechocystis sp. strain PCC 6803 is involved in natural transformation, phototactic motility and piliation.
pubmed:affiliation
School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't