Source:http://linkedlifedata.com/resource/pubmed/id/17156791
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2007-1-8
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pubmed:abstractText |
A novel technique based on dynamic microwave-assisted extraction (DMAE) coupled on-line with high-performance liquid chromatography (HPLC) through a flow injection interface has been developed for determination of andrographolide and dehydroandrographolide in Andrographis paniculata Nees. A TM(010) microwave resonance cavity built in the laboratory was applied to concentrating the microwave energy. An extraction vessel was placed in microwave irradiation zone. The extraction was performed in a recirculating system. When a number of extraction cycles were completed, the fractional extract (20muL) was driven to the analytical column by 65% aqueous methanol and was measured by diode array detector (DAD) at 225nm. The optimized extraction conditions are follows: extraction solvent 60% aqueous methanol; microwave forward power 80W; extraction time 6min; extraction solvent flow-rate 1.0mLmin(-1). The detection and quantification limits obtained are 0.5 and 1.7microgmL(-1) for andrographolide and 0.6 and 1.9microgmL(-1) for dehydroandrographolide, respectively. The within-day and between-day precision (RSD) are 2.1% and 3.7% for andrographolide and 1.7% and 4.1% for dehydroandrographolide, respectively. Mean recoveries for andrographolide and dehydroandrographolide are 97.7% and 98.7%, respectively. Compared with ultrasonic extraction used in the Chinese pharmacopoeia, the proposed method was demonstrated to obtain higher extraction yield in a shorter time. In addition, only small quantities of solvent (5mL) and sample (10mg) were required.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Diterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Methanol,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents,
http://linkedlifedata.com/resource/pubmed/chemical/andrographolide,
http://linkedlifedata.com/resource/pubmed/chemical/dehydroandrographolide
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
1140
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-7
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pubmed:dateRevised |
2009-1-15
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pubmed:meshHeading |
pubmed-meshheading:17156791-Andrographis,
pubmed-meshheading:17156791-Automation,
pubmed-meshheading:17156791-Chemical Fractionation,
pubmed-meshheading:17156791-Chromatography, High Pressure Liquid,
pubmed-meshheading:17156791-Diterpenes,
pubmed-meshheading:17156791-Methanol,
pubmed-meshheading:17156791-Microwaves,
pubmed-meshheading:17156791-Reproducibility of Results,
pubmed-meshheading:17156791-Solvents,
pubmed-meshheading:17156791-Ultrasonics
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pubmed:year |
2007
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pubmed:articleTitle |
On-line coupling of dynamic microwave-assisted extraction with high-performance liquid chromatography for determination of andrographolide and dehydroandrographolide in Andrographis paniculata Nees.
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pubmed:affiliation |
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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