Linked Life Data

17156358

Source:http://linkedlifedata.com/resource/pubmed/id/17156358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-12-12
pubmed:abstractText
It is widely believed that the pyramidal cells and interneurons of the cerebral cortex are distinct in their origin, lineage and genetic make up. In view of these findings, the current thesis is that the phenotype determination of cortical neurons is primarily directed by genetic mechanisms. Using in vitro assays, the present study demonstrates that secreted factors from ganglionic eminence (GE) of the ventral telencephalon have the potency to induce the differentiation of a subset of cortical neurons towards gamma-aminobutyric acid (GABA)ergic lineage. Characterization of cortical cultures that were exposed to medium derived from GE illustrated a significant increase in the number of GABA-, calretinin- and calbindin-positive neurons. Calcium imaging together with pharmacological studies showed that the application of exogenous medium significantly elevated the intracellular calcium transients in cortical neurons through the activation of ionotropic glutamate receptors. The increase in GABA+ neurons appeared to be associated with the elevated calcium activity; treatment with blockers specific for glutamate receptors abolished both the synchronized transients and reduced the differentiation of GABAergic neurons. Such studies demonstrate that although intrinsic mechanisms determine the fate of cortical interneurons, extrinsic factors have the potency to influence their neurochemical differentiation and contribute towards their molecular diversity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2967-77
pubmed:dateRevised
2007-8-13
pubmed:meshHeading
pubmed-meshheading:17156358-Animals, pubmed-meshheading:17156358-Biological Markers, pubmed-meshheading:17156358-Calcium, pubmed-meshheading:17156358-Calcium Signaling, pubmed-meshheading:17156358-Calcium-Binding Proteins, pubmed-meshheading:17156358-Cell Differentiation, pubmed-meshheading:17156358-Cell Lineage, pubmed-meshheading:17156358-Cell Movement, pubmed-meshheading:17156358-Cells, Cultured, pubmed-meshheading:17156358-Cerebral Cortex, pubmed-meshheading:17156358-Culture Media, Conditioned, pubmed-meshheading:17156358-Excitatory Amino Acid Antagonists, pubmed-meshheading:17156358-Interneurons, pubmed-meshheading:17156358-Membrane Potentials, pubmed-meshheading:17156358-Nerve Growth Factors, pubmed-meshheading:17156358-Phenotype, pubmed-meshheading:17156358-Rats, pubmed-meshheading:17156358-Rats, Sprague-Dawley, pubmed-meshheading:17156358-Receptors, Glutamate, pubmed-meshheading:17156358-Stem Cells, pubmed-meshheading:17156358-Telencephalon, pubmed-meshheading:17156358-gamma-Aminobutyric Acid
pubmed:year
2006
pubmed:articleTitle
Secreted factors from ventral telencephalon induce the differentiation of GABAergic neurons in cortical cultures.
pubmed:affiliation
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't