| pubmed-article:1715528 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1715528 | lifeskim:mentions | umls-concept:C0205463 | lld:lifeskim |
| pubmed-article:1715528 | lifeskim:mentions | umls-concept:C0205464 | lld:lifeskim |
| pubmed-article:1715528 | lifeskim:mentions | umls-concept:C0994894 | lld:lifeskim |
| pubmed-article:1715528 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:1715528 | pubmed:dateCreated | 1991-9-30 | lld:pubmed |
| pubmed-article:1715528 | pubmed:abstractText | Whole cell and patch clamp techniques were used to investigate the properties of 5-HT3 receptors of a murine neuroblastoma cell line (N1E-115) and adult rabbit nodose ganglion neurones. In addition, some preliminary results from guinea-pig nodose ganglion neurones are presented. In such cells, voltage-clamped at -60 mV, 5-HT (10 microM) induced an inward current associated with a conductance increase. The results of ion substitution experiments suggest that the 5-HT activated ion channel is permeable to both Na+ and K+ ions with a permeability ratio (PNa/PK) of 0.94 and 0.92 for rabbit nodose ganglion cells and N1E-115 cells respectively. On outside out membrane patches excised from rabbit nodose ganglion neurones, 5-HT (1 microM) activated clearly discernible single channel currents with a conductance of 16.6 +/- 0.7 pS (n = 4). In contrast, fluctuation analysis of 5-HT induced whole cell currents suggests that the single channel conductance of N1E-115 cells is only 0.3 pS, a value some 50 fold lower. The 5-HT-induced whole cell currents recorded from all three preparations were antagonised by the selective 5-HT3 receptor antagonist ondansetron (GR38032F) and by the less selective agents metoclopramide, cocaine and (+)-tubocurarine. However, these preparations demonstrate a differential sensitivity to some antagonists. In particular, (+)-tubocurarine was a potent antagonist in N1E-115 cells (IC50 = 0.85 nM) but was approximately 200 fold (IC50 = 156 nM) and 1200 fold (IC50 = 10 microM) less potent in rabbit and guinea-pig nodose ganglion neurones respectively. Additionally, a novel effect of ketamine (10 microM) to potentiate the 5-HT-induced current of rabbit nodose ganglion neurones is described. | lld:pubmed |
| pubmed-article:1715528 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1715528 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1715528 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1715528 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1715528 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1715528 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1715528 | pubmed:issn | 0143-4179 | lld:pubmed |
| pubmed-article:1715528 | pubmed:author | pubmed-author:PetersJ AJA | lld:pubmed |
| pubmed-article:1715528 | pubmed:author | pubmed-author:LambertJ JJJ | lld:pubmed |
| pubmed-article:1715528 | pubmed:author | pubmed-author:MaloneH MHM | lld:pubmed |
| pubmed-article:1715528 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1715528 | pubmed:volume | 19 Suppl | lld:pubmed |
| pubmed-article:1715528 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1715528 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1715528 | pubmed:pagination | 25-30 | lld:pubmed |
| pubmed-article:1715528 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
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| pubmed-article:1715528 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1715528 | pubmed:articleTitle | Physiological and pharmacological properties of 5-HT3 receptors--a patch clamp-study. | lld:pubmed |
| pubmed-article:1715528 | pubmed:affiliation | Department of Pharmacology and Clinical Pharmacology, Ninewells Hospital and Medical School, University of Dundee. | lld:pubmed |
| pubmed-article:1715528 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1715528 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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