Linked Life Data

1715528

Source:http://linkedlifedata.com/resource/pubmed/id/1715528

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-9-30
pubmed:abstractText
Whole cell and patch clamp techniques were used to investigate the properties of 5-HT3 receptors of a murine neuroblastoma cell line (N1E-115) and adult rabbit nodose ganglion neurones. In addition, some preliminary results from guinea-pig nodose ganglion neurones are presented. In such cells, voltage-clamped at -60 mV, 5-HT (10 microM) induced an inward current associated with a conductance increase. The results of ion substitution experiments suggest that the 5-HT activated ion channel is permeable to both Na+ and K+ ions with a permeability ratio (PNa/PK) of 0.94 and 0.92 for rabbit nodose ganglion cells and N1E-115 cells respectively. On outside out membrane patches excised from rabbit nodose ganglion neurones, 5-HT (1 microM) activated clearly discernible single channel currents with a conductance of 16.6 +/- 0.7 pS (n = 4). In contrast, fluctuation analysis of 5-HT induced whole cell currents suggests that the single channel conductance of N1E-115 cells is only 0.3 pS, a value some 50 fold lower. The 5-HT-induced whole cell currents recorded from all three preparations were antagonised by the selective 5-HT3 receptor antagonist ondansetron (GR38032F) and by the less selective agents metoclopramide, cocaine and (+)-tubocurarine. However, these preparations demonstrate a differential sensitivity to some antagonists. In particular, (+)-tubocurarine was a potent antagonist in N1E-115 cells (IC50 = 0.85 nM) but was approximately 200 fold (IC50 = 156 nM) and 1200 fold (IC50 = 10 microM) less potent in rabbit and guinea-pig nodose ganglion neurones respectively. Additionally, a novel effect of ketamine (10 microM) to potentiate the 5-HT-induced current of rabbit nodose ganglion neurones is described.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0143-4179
pubmed:author
pubmed:issnType
Print
pubmed:volume
19 Suppl
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-30
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed-meshheading:1715528-Action Potentials, pubmed-meshheading:1715528-Animals, pubmed-meshheading:1715528-Cocaine, pubmed-meshheading:1715528-Ganglia, pubmed-meshheading:1715528-Guinea Pigs, pubmed-meshheading:1715528-Imidazoles, pubmed-meshheading:1715528-Ion Channels, pubmed-meshheading:1715528-Ketamine, pubmed-meshheading:1715528-Metoclopramide, pubmed-meshheading:1715528-Neuroblastoma, pubmed-meshheading:1715528-Neurons, pubmed-meshheading:1715528-Ondansetron, pubmed-meshheading:1715528-Potassium, pubmed-meshheading:1715528-Rabbits, pubmed-meshheading:1715528-Receptors, Serotonin, pubmed-meshheading:1715528-Serotonin, pubmed-meshheading:1715528-Serotonin Antagonists, pubmed-meshheading:1715528-Sodium, pubmed-meshheading:1715528-Tubocurarine, pubmed-meshheading:1715528-Tumor Cells, Cultured
pubmed:year
1991
pubmed:articleTitle
Physiological and pharmacological properties of 5-HT3 receptors--a patch clamp-study.
pubmed:affiliation
Department of Pharmacology and Clinical Pharmacology, Ninewells Hospital and Medical School, University of Dundee.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't