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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1991-10-2
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pubmed:abstractText |
Primary neurons in culture from chick embryo cerebral hemispheres were treated with a mixture of gangliosides added to the growth medium (final concentration: 10(-5)M and 10(-8)M) from the 3rd to the 6th day in vitro. Under these conditions methylation processes measured with [3H] and [35S] methionine and [3H]ethanolamine as precursors showed an increased methylation of [3H]ethanolamine containing phospholipids, a correspondent increased conversion of these compounds to [3H]choline containing phospholipids, and a general increased methylation of trichloroacetic acid precipitable macromolecules containing labeled methionine. A small increase in protein synthesis was observed after incubation of neurons with [3H]- and [35S]methionine. This was confirmed after electrophoretic separation of a protein extract with increased 3H- and 35S-labeling in protein bands with moecular weights between 50 and 60 KDaltons. A protein band of about 55 KDaltons appeared to be preferentially labelled when [3H] methionine was the precursor. The treatment with gangliosides increased the incorporation of [methyl-3H] label after incubation of neurons with [3H] methionine, into total DNA and decreased that of total RNA. The treatment of neurons in culture with exogenous gangliosides hence affects differently methylation processes, a finding which may confirm the involvement of gangliosides on the intracellular mediation of neuronal information mechanisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanolamine,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Gangliosides,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/S-Adenosylmethionine,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Trichloroacetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0364-3190
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
137-44
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1715527-Animals,
pubmed-meshheading:1715527-Brain,
pubmed-meshheading:1715527-Cells, Cultured,
pubmed-meshheading:1715527-Chemical Precipitation,
pubmed-meshheading:1715527-Chick Embryo,
pubmed-meshheading:1715527-Choline,
pubmed-meshheading:1715527-DNA,
pubmed-meshheading:1715527-Ethanolamine,
pubmed-meshheading:1715527-Ethanolamines,
pubmed-meshheading:1715527-Gangliosides,
pubmed-meshheading:1715527-Methionine,
pubmed-meshheading:1715527-Methylation,
pubmed-meshheading:1715527-Molecular Weight,
pubmed-meshheading:1715527-Neurons,
pubmed-meshheading:1715527-Phospholipids,
pubmed-meshheading:1715527-RNA,
pubmed-meshheading:1715527-S-Adenosylmethionine,
pubmed-meshheading:1715527-Sulfur Radioisotopes,
pubmed-meshheading:1715527-Trichloroacetic Acid,
pubmed-meshheading:1715527-Tritium
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pubmed:year |
1991
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pubmed:articleTitle |
Exogenous gangliosides may affect methylation mechanisms in neuronal cell cultures.
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pubmed:affiliation |
Centre de Neurochimie du CNRS, Strasbourg, France.
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pubmed:publicationType |
Journal Article
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