Linked Life Data

17150613

Source:http://linkedlifedata.com/resource/pubmed/id/17150613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
49
pubmed:dateCreated
2006-12-7
pubmed:abstractText
3-Deazaguanine (3DG) and its 2-N-acylated derivatives were incorporated into 2'-O-methyl-RNAs, and the hybridization and base discrimination properties were clarified by measuring UV-melting profiles. The 2'-O-methyl-RNA incorporating a 3DG hybridized less strongly in comparison to that without 3-deaza modification to both fully-matched and singly mismatched target strands. The T(m) decrease was the largest in the case of the fully matched duplex so that the base discrimination ability was decreased. Interestingly, the acetylation of the amino group of 3DG could compensate the T(m) decrease of the fully matched duplex by 3DG. Moreover, it was also proved that the base pair of 2-N-acetyl-3-deazaguanine and uracil was much weaker than the G-U pair. These results indicate the possibility of the development of guanine analogs that can recognize cytosine more precisely than guanine by use of the 2-N-acyl-3-deazaguanine skeleton.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1746-8272
pubmed:author
pubmed:issnType
Electronic
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-2
pubmed:dateRevised
2007-9-19
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Synthesis and hybridization properties of 2'-O-methyl-RNA incorporating 3-deazaguanine derivatives.
pubmed:affiliation
Frontier Collaborative Research Center, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
pubmed:publicationType
Journal Article