|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
50
|
|
pubmed:dateCreated |
2006-12-13
|
|
pubmed:abstractText |
We report the engineering of the surface of the tobacco mosaic virus (TMV) virion with a mosquito decapeptide hormone, trypsin-modulating oostatic factor (TMOF). The TMV coat protein (CP) was fused to TMOF at the C terminus by using a read-through, leaky stop codon that facilitated expression of CP and chimeric CP-TMOF (20:1 ratio) that were coassembled into virus particles in infected Nicotiana tabacum. Plants that were infected with the hybrid TMV RNA accumulated TMOF to levels of 1.3% of total soluble protein. Infected tobacco leaf discs that were fed to Heliothis virescens fourth-instar larvae stunted their growth and inhibited trypsin and chymotrypsin activity in their midgut. Purified CP-TMOF virions fed to mosquito larvae stopped larval growth and caused death. Because TMV has a wide host range, expressing TMV-TMOF in plants can be used as a general method to protect them against agricultural insect pests and to control vector mosquitoes.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-10069957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-10411621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-11462221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-1421442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-14609198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-14871616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-14976987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-14981657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-15082933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-1546328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-1651497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-16593669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-2394318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-2602379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-2979536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-3418781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-3532863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-7480877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-7763916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-7908271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-8159807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-8353526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-8446627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-8512567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-8620885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17148608-9634749
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
12
|
|
pubmed:volume |
103
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
18963-8
|
|
pubmed:dateRevised |
2010-9-15
|
|
pubmed:meshHeading |
pubmed-meshheading:17148608-Aedes,
pubmed-meshheading:17148608-Animal Feed,
pubmed-meshheading:17148608-Animals,
pubmed-meshheading:17148608-Capsid Proteins,
pubmed-meshheading:17148608-Genome, Viral,
pubmed-meshheading:17148608-Insecticides,
pubmed-meshheading:17148608-Larva,
pubmed-meshheading:17148608-Models, Molecular,
pubmed-meshheading:17148608-Oligopeptides,
pubmed-meshheading:17148608-Plant Diseases,
pubmed-meshheading:17148608-Plant Leaves,
pubmed-meshheading:17148608-Protein Engineering,
pubmed-meshheading:17148608-Protein Structure, Tertiary,
pubmed-meshheading:17148608-Recombinant Fusion Proteins,
pubmed-meshheading:17148608-Tobacco,
pubmed-meshheading:17148608-Tobacco Mosaic Virus,
pubmed-meshheading:17148608-Trypsin,
pubmed-meshheading:17148608-Virion
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Expression of Aedes trypsin-modulating oostatic factor on the virion of TMV: A potential larvicide.
|
|
pubmed:affiliation |
Florida Medical Entomology Laboratory, University of Florida-Institute of Food and Agricultural Sciences (IFAS), 200 Ninth Street Southeast, Vero Beach, FL 32962-4699, USA. dobo@mail.ifas.ufl.edu
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
