Linked Life Data

17148583

Source:http://linkedlifedata.com/resource/pubmed/id/17148583

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2007-5-9
pubmed:abstractText
Many members of the nearly 200-strong homeobox gene family have been implicated in cancer, mostly following ectopic expression. In this study we analyzed homeobox gene expression in Hodgkin lymphoma (HL) cell lines. Both reverse transcription-polymerase chain reaction (RT-PCR) using degenerate primers and microarray profiling identified consistently up-regulated HOXB9 expression. Analysis of HOXB9 regulation in HL cells revealed E2F3A and BMI1 as activator and repressor, respectively. Furthermore, a constitutively active ERK5 pathway was identified in all HL cell lines analyzed as well as primary HL cells. Our data show that ERK5 probably mediates HOXB9 expression by repressing BMI1. In addition, expression analysis of the neighboring microRNA gene mir-196a1 revealed coregulation with HOXB9. Functional analysis of HOXB9 by knockdown and overexpression assays indicated their influence on both proliferation and apoptosis in HL cells. In summary, we identified up-regulation of HOXB9 in HL mediated by constitutively active ERK5 signaling which may represent novel therapeutic targets in HL.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3015-23
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17148583-Base Sequence, pubmed-meshheading:17148583-Cell Line, Tumor, pubmed-meshheading:17148583-Gene Expression Profiling, pubmed-meshheading:17148583-Gene Expression Regulation, Neoplastic, pubmed-meshheading:17148583-Genes, Homeobox, pubmed-meshheading:17148583-Hodgkin Disease, pubmed-meshheading:17148583-Homeodomain Proteins, pubmed-meshheading:17148583-Humans, pubmed-meshheading:17148583-MAP Kinase Signaling System, pubmed-meshheading:17148583-Mitogen-Activated Protein Kinase 7, pubmed-meshheading:17148583-Multigene Family, pubmed-meshheading:17148583-Nuclear Proteins, pubmed-meshheading:17148583-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17148583-Proto-Oncogene Proteins, pubmed-meshheading:17148583-RNA, Small Interfering, pubmed-meshheading:17148583-Repressor Proteins, pubmed-meshheading:17148583-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2007
pubmed:articleTitle
Comprehensive analysis of homeobox genes in Hodgkin lymphoma cell lines identifies dysregulated expression of HOXB9 mediated via ERK5 signaling and BMI1.
pubmed:affiliation
Human and Animal Cell Cultures, Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ), Braunschweig, Germany. sna@dsmz.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't