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rdf:type |
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lifeskim:mentions |
umls-concept:C0021528,
umls-concept:C0021920,
umls-concept:C0035668,
umls-concept:C0039679,
umls-concept:C0205145,
umls-concept:C0220781,
umls-concept:C0332256,
umls-concept:C0441843,
umls-concept:C0887917,
umls-concept:C0936012,
umls-concept:C1514873,
umls-concept:C1519249,
umls-concept:C1883254
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pubmed:issue |
15
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pubmed:dateCreated |
1991-9-18
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pubmed:abstractText |
Two protected derivatives of the ribonucleoside inosine have been prepared to serve as building blocks for phosphoramidite-based synthesis of RNA. Two different synthetic routes address the unusual solubility characteristics of inosine and its derivatives. The final products of the different synthetic pathways, 5'-O-(dimethoxytrityl)-2'-O-(t-butyldimethylsiyl) inosine 3'-O-(beta-cyanoethyldiisopropylamino) phosphoramidite 5a, and O6-p-nitrophenylethyl-5'-O-(dimethoxytrityl)-2'-O-(t-butyldimethylsilyl) inosine 3'-O-(methyldiisopropylamino) phosphoramidite 5b, were chemically incorporated into short oligoribonucleotides which also contained the four standard ribonucleoside bases. The oligomers were chosen to study base-specific interactions between an RNA substrate and an RNA enzyme derived from the Group I Tetrahymena self-splicing intron. The oligomers were shown to be biochemically competent using a trans cleavage assay with the modified Tetrahymena intron. The results confirm the dependence of the catalytic activity on a wobble base pair, rather than a Watson-Crick base pair, in the helix at the 5'-splice site. Furthermore, comparison of guanosine and inosine in a wobble base pair allows one to assess the importance of the guanine 2-amino group for biological activity. The preparation of the inosine phosphoramidites adds to the repertoire of base analogues available for the study of RNA catalysis and RNA-protein interactions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-11540922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-1700131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-1986368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2216717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2258934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2452483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2585502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2660003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2678103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-2911488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-3285220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-3413059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-3684574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-3748816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-3783714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-3838308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-4080553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-5969078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1714564-6728676
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4161-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1714564-Animals,
pubmed-meshheading:1714564-Base Composition,
pubmed-meshheading:1714564-Base Sequence,
pubmed-meshheading:1714564-DNA Mutational Analysis,
pubmed-meshheading:1714564-Inosine,
pubmed-meshheading:1714564-Introns,
pubmed-meshheading:1714564-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1714564-Molecular Sequence Data,
pubmed-meshheading:1714564-RNA,
pubmed-meshheading:1714564-RNA Splicing,
pubmed-meshheading:1714564-Tetrahymena
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pubmed:year |
1991
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pubmed:articleTitle |
Synthesis of RNA containing inosine: analysis of the sequence requirements for the 5' splice site of the Tetrahymena group I intron.
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pubmed:affiliation |
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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