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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2006-12-6
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pubmed:abstractText |
Activation-induced cytidine deaminase (AID) likely initiates immunoglobulin gene-conversion (GC) by deaminating cytidines within the V-region of chicken B-cells. However, the intervening DNA lesion required to initiate GC remains elusive. GC could be initiated by a single strand break or a double strand break (DSB). To distinguish between these possibilities, we examined GC in the chicken DT40 B cell line deficient in non-homologous end joining (NHEJ). It is known that the NHEJ and homologous recombination DNA repair pathways compete for DSBs. In light of this, if a DSB is the major intermediate, deficiency in NHEJ should result in increased levels of GC. Here we show that DNA-PKcs(-/-/-) and Ku70(-/-) DT40 cells had 5- to 10-fold higher levels of GC relative to wildtype DT40 as measured by surface IgM reversion and sequencing of the V-region. These data suggest that a DSB is the major DNA lesion that initiates GC.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-10373455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11007474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11074004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11283264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11333245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11526098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11528482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11717442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11740565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11751629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11823785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11847344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11882297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-11994424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12097915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12154379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12401169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12461083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12633669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12651944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12818155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-12897142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-15039385,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-15229473,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-16464008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-16498404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-16807135,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-8016116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-9099798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-9435225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-9625768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17142237-9736627
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6345-51
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17142237-Animals,
pubmed-meshheading:17142237-Antigens, Nuclear,
pubmed-meshheading:17142237-Cell Line,
pubmed-meshheading:17142237-Chickens,
pubmed-meshheading:17142237-DNA Breaks, Double-Stranded,
pubmed-meshheading:17142237-DNA Repair,
pubmed-meshheading:17142237-DNA-Activated Protein Kinase,
pubmed-meshheading:17142237-DNA-Binding Proteins,
pubmed-meshheading:17142237-Gene Conversion,
pubmed-meshheading:17142237-Gene Deletion,
pubmed-meshheading:17142237-Genes, Immunoglobulin,
pubmed-meshheading:17142237-Immunoglobulin M
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pubmed:year |
2006
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pubmed:articleTitle |
NHEJ-deficient DT40 cells have increased levels of immunoglobulin gene conversion: evidence for a double strand break intermediate.
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pubmed:affiliation |
Department of Immunology, 5265 Medical Sciences Building, University of Toronto, Ontario, Toronto, Canada M5S 1A8.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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