Linked Life Data

17136369

Source:http://linkedlifedata.com/resource/pubmed/id/17136369

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-2-23
pubmed:abstractText
The aim of this work was to evaluate phytohormone biosynthesis, siderophores production, and phosphate solubilization in three strains (E109, USDA110, and SEMIA5080) of Bradyrhizobium japonicum, most commonly used for inoculation of soybean and nonlegumes in USA, Canada, and South America. Siderophore production and phosphate solubilization were evaluated in selective culture conditions, which had negative results. Indole-3-acetic acid (IAA), gibberellic acid (GA(3)), and abscisic acid (ABA) production were analyzed by gas chromatography-mass spectrometry (GC-MS). Ethylene and zeatin biosynthesis were determined by GS-flame ionization detection and high-performance liquid chromatography (HPLC-UV), respectively. IAA, zeatin, and GA(3) were found in all three strains; however, their levels were significantly higher (p < 0.01) in SEMIA5080 (3.8 microg ml(-1)), USDA110 (2.5 microg ml(-1)), and E109 (0.87 microg ml(-1)), respectively. ABA biosynthesis was detected only in USDA110 (0.019 microg ml(-1)). Ethylene was found in all three strains, with highest production rate (18.1 ng ml(-1) h(-1)) in E109 cultured in yeast extract mannitol medium plus L-methionine. This is the first report of IAA, GA(3), zeatin, ethylene, and ABA production by B. japonicum in pure cultures, using quantitative physicochemical methodology. The three strains have differential capability to produce the five major phytohormones and this fact may have an important technological implication for inoculant formulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0175-7598
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
874-80
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Phytohormone production by three strains of Bradyrhizobium japonicum and possible physiological and technological implications.
pubmed:affiliation
Laboratorio de Fisiología Vegetal, Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Campus Universitario, CP 5800, Río Cuarto, Córdoba, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't