Linked Life Data

1713511

Source:http://linkedlifedata.com/resource/pubmed/id/1713511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1991-9-4
pubmed:abstractText
Colony-stimulating factors (CSF) are important factors in the proliferation and differentiation of hematopoietic progenitor cells (HPC), and in the survival and activation of mature blood cells. Interleukin-1 (IL-1) combined with fetal bovine serum (FBS) strongly induces the expression of macrophage-CSF (M-CSF), granulocyte-CSF (G-CSF), and granulocyte-macrophage-CSF (GM-CSF) in fibroblasts. Here, we report on the regulation of CSF gene expression in murine fibroblasts following IL-1 and FBS stimulation. We demonstrate that 10T1/2 murine fibroblasts induced by FBS or IL-1 accumulate M-CSF messenger RNA (mRNA). G-CSF mRNA expression was induced by IL-1, and not by FBS. For GM-CSF expression, induction with both FBS and IL-1 was required. Blocking studies with actinomycin-D showed that active transcription is essential for accumulation of all three CSF mRNAs. After blocking protein synthesis with cycloheximide, IL-1- or FBS-induced M-CSF expression and IL-1 plus FBS-induced GM-CSF expression still occurred and was increased. IL-1-induced G-CSF expression was completely prevented in these cells by pretreatment with cycloheximide, illustrating that, for this effect, intermediate protein synthesis was required. The half-lives of M-CSF transcripts were not substantially altered by addition of IL-1, FBS, or FBS plus IL-1. Using nuclear run-on assays, we demonstrated that the transcription rate of M-CSF was increased up to 20-fold by the addition of FBS, IL-1, or FBS plus IL-1. After blocking protein synthesis with cycloheximide, IL-1-or FBS-induced increase in M-CSF transcription rate was also observed. GM-CSF transcription increased up to fourfold after induction with FBS or IL-1. G-CSF transcription rate was not altered by FBS or IL-1. Our results indicate that M-CSF expression induced by FBS or IL-1 in these fibroblasts is primarily regulated at the transcriptional level. GM-CSF expression appears to be regulated both transcriptionally and posttranscriptionally, and G-CSF expression is regulated mainly at the posttranscriptional level.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
658-65
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1713511-Animals, pubmed-meshheading:1713511-Blood, pubmed-meshheading:1713511-Cattle, pubmed-meshheading:1713511-Cell Line, pubmed-meshheading:1713511-Cell Nucleus, pubmed-meshheading:1713511-Colony-Stimulating Factors, pubmed-meshheading:1713511-Culture Media, pubmed-meshheading:1713511-Cycloheximide, pubmed-meshheading:1713511-Dactinomycin, pubmed-meshheading:1713511-Fetus, pubmed-meshheading:1713511-Fibroblasts, pubmed-meshheading:1713511-Gene Expression Regulation, pubmed-meshheading:1713511-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:1713511-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:1713511-Interleukin-1, pubmed-meshheading:1713511-Kinetics, pubmed-meshheading:1713511-Macrophage Colony-Stimulating Factor, pubmed-meshheading:1713511-Mice, pubmed-meshheading:1713511-Protein Biosynthesis, pubmed-meshheading:1713511-RNA, Messenger, pubmed-meshheading:1713511-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
Differential transcriptional and posttranscriptional regulation of gene expression of the colony-stimulating factors by interleukin-1 and fetal bovine serum in murine fibroblasts.
pubmed:affiliation
Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't