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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-11-29
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pubmed:abstractText |
An anti-Tat hairpin ribozyme and a TAR RNA decoy were combined in one molecule. The chimeric molecule strongly inhibited HIV-1 replication (measured as changes in p24 levels in viral replication assays). The inhibitory action of the ribodecozyme (85%) was significantly greater than that shown by ribozyme and a non-catalytic variant carrying the functional decoy RNA domain (55% and 35%, respectively). This represents a significant improvement of the inhibitory efficiency of the ribozyme, suggesting there is an additive inhibitory effect on HIV-1 replication by the catalytic and decoy domains. This strategy could be used to create new inhibitor RNAs with enhanced in vivo performance.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1555-8584
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-9
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pubmed:dateRevised |
2010-1-15
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pubmed:meshHeading |
pubmed-meshheading:17132944-Base Sequence,
pubmed-meshheading:17132944-Cells, Cultured,
pubmed-meshheading:17132944-Drug Design,
pubmed-meshheading:17132944-Gene Products, tat,
pubmed-meshheading:17132944-HIV-1,
pubmed-meshheading:17132944-Humans,
pubmed-meshheading:17132944-Molecular Sequence Data,
pubmed-meshheading:17132944-RNA, Catalytic,
pubmed-meshheading:17132944-RNA, Viral,
pubmed-meshheading:17132944-Virus Replication,
pubmed-meshheading:17132944-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2005
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pubmed:articleTitle |
Inhibition of HIV-1 replication by an improved hairpin ribozyme that includes an RNA decoy.
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pubmed:affiliation |
Instituto de Parasitología y Biomedicina López-Neyra, CSIC, Parque Tecnológico de Ciencias de la Salud, Avda, del Conocimiento s/n, Granada, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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