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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1991-8-28
|
| pubmed:abstractText |
Brain astrocytes were established in primary culture from postnatal and adult rats to characterize the developmental expression of secreted proteins. Astrocytes cultured from 21-day rat brain, but not 1-day rat brain, secreted a distinct group of proteins with Mr of 35,000 as determined by analysis of [35S]methionine-labeled proteins using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. N-terminal amino acid sequence analysis of this protein group showed 100% identity to rat insulin-like growth factor binding protein-2 (rIGFBP-2), the BRL-3A IGFBP purified from a fetal rat liver cell line. An antiserum was generated against this astrocyte 35,000 Mr protein, and immunoblot analysis revealed a dramatic increase in rIGFBP-2 secretion in astrocytes cultured from 14-day, 21-day, and adult rat brain compared to astrocytes from 1-day and 7-day rat brain. Similar analysis of neonatal rat brain neurons in culture failed to show immunoreactive rIGFBP-2 in cell lysates or secreted protein. Ligand Western blot analysis demonstrated [125I]IGF-II binding to a single protein band which comigrated with a prominant rIGFBP-2 immunoreactive species in nonreduced conditioned medium from 21-day astrocytes. In comparison [125I]IGF-II binding proteins were detected only at low levels in medium from astrocytes cultured from 1-day rat brain and were undetectable in neuron-conditioned media. Northern blot analysis using a rIGFBP-2 complementary DNA revealed 5-fold greater messenger RNA levels in astrocytes from 21-day rat brain compared with astrocytes from 1-day brain, whereas neonate neurons showed no transcripts. Thus, rIGFBP-2 exhibits a pattern of developmental and cell-specific expression in cultured rat brain cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor II,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0013-7227
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
129
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1066-74
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1713158-Aging,
pubmed-meshheading:1713158-Amino Acid Sequence,
pubmed-meshheading:1713158-Animals,
pubmed-meshheading:1713158-Animals, Newborn,
pubmed-meshheading:1713158-Astrocytes,
pubmed-meshheading:1713158-Brain,
pubmed-meshheading:1713158-Carrier Proteins,
pubmed-meshheading:1713158-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1713158-Immunoblotting,
pubmed-meshheading:1713158-Insulin-Like Growth Factor Binding Protein 2,
pubmed-meshheading:1713158-Insulin-Like Growth Factor II,
pubmed-meshheading:1713158-Isoelectric Point,
pubmed-meshheading:1713158-Molecular Sequence Data,
pubmed-meshheading:1713158-Molecular Weight,
pubmed-meshheading:1713158-Nucleic Acid Hybridization,
pubmed-meshheading:1713158-RNA, Messenger,
pubmed-meshheading:1713158-Rats,
pubmed-meshheading:1713158-Rats, Inbred Strains
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Developmental expression of rat insulin-like growth factor binding protein-2 by astrocytic glial cells in culture.
|
| pubmed:affiliation |
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville 32610.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|