Linked Life Data

17131336

Source:http://linkedlifedata.com/resource/pubmed/id/17131336

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-1-2
pubmed:abstractText
Melanoma-associated retinopathy (MAR) is a rare paraneoplastic syndrome in patients with melanoma. Since the onset of MAR symptoms is often associated with tumor progression or recrudescence of metastases, MAR-related symptoms are prognostic relevant. The pathomechanism underlying MAR is supposed to result from antibody production against yet unknown melanoma-associated antigens that are also expressed in retinal tissue, leading to the destruction of retinal cells and resulting in defective signal transduction. Only a 35 kDa protein in Müller glial cells, a 22 kDa neuronal antigen and retinal transducin have been identified as MAR-associated antigens to date. To identify additional antigens potentially involved in the pathogenesis of MAR, we screened a retina cDNA phage library for reactivity with antibodies in the sera from 9 patients with MAR or subclinical MAR using the serological analysis of recombinantly expressed clones (SEREX) approach. Six sera from melanoma patients without evidence of MAR and 10 sera from healthy donors served as controls. Mitofilin and titin were identified as antigens against which antibodies were found exclusively in sera of MAR patients, but not in the sera of MM patients without MAR or healthy donors. This is the first study to demonstrate that titin is highly expressed from retinal tissue and melanoma. The fact that none of the MAR-associated antigens detected to date by their capacity to elicit a humoral immune response is located on the cell surface questions a major pathogenetic role of the respective antibodies and suggests that cellular, rather than humoral mechanisms are operative in the primary immune attack against the retina in MAR.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
120
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
788-95
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17131336-Antibodies, Neoplasm, pubmed-meshheading:17131336-Antigens, Neoplasm, pubmed-meshheading:17131336-Blotting, Northern, pubmed-meshheading:17131336-Cell Line, Tumor, pubmed-meshheading:17131336-DNA, Complementary, pubmed-meshheading:17131336-DNA, Neoplasm, pubmed-meshheading:17131336-Gene Library, pubmed-meshheading:17131336-Humans, pubmed-meshheading:17131336-Immunoenzyme Techniques, pubmed-meshheading:17131336-Melanoma, pubmed-meshheading:17131336-Mitochondrial Proteins, pubmed-meshheading:17131336-Muscle Proteins, pubmed-meshheading:17131336-Paraneoplastic Syndromes, pubmed-meshheading:17131336-Peptide Library, pubmed-meshheading:17131336-Protein Kinases, pubmed-meshheading:17131336-RNA, Neoplasm, pubmed-meshheading:17131336-Retinal Diseases, pubmed-meshheading:17131336-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17131336-Serologic Tests, pubmed-meshheading:17131336-Skin Neoplasms
pubmed:year
2007
pubmed:articleTitle
Mitofilin and titin as target antigens in melanoma-associated retinopathy.
pubmed:affiliation
Department of Dermatology, Saarland University Medical School, Homburg/Saar, Germany. hacpfo@uniklinik-saarland.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't