Source:http://linkedlifedata.com/resource/pubmed/id/17130829
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
23
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pubmed:dateCreated |
2007-5-17
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pubmed:abstractText |
Zinc-finger protein 217 (ZNF217) is a Kruppel-like zinc-finger protein located at 20q13.2, within a region of recurrent maximal amplification. Here, we demonstrate that ZNF217 is a transcriptional repressor protein and report the purification and characterization of a ZNF217 complex. The purified ZNF217 complex consists of approximately six proteins and contains the transcriptional co-repressors CoREST, BHC110/LSD1, histone deacetylase (HDAC) 2 and C-terminal binding protein (CtBP1). The purified ZNF217 complex possesses deacetylase activity as well as lysine 4 histone H3-specific demethylase activity that is most likely mediated by the BHC110/LSD1 component. To determine if ZNF217 is a sequence-specific binding protein, we have made use of cyclic amplification and selection of targets (CAST) assay and identify for the first time a ZNF217 DNA consensus recognition sequence (CRS) that is highly conserved in the human E-cadherin promoter. Chromatin immunoprecipitation (ChIP) experiments demonstrate that ZNF217, as well as the other components of the ZNF217 complex, are found on the region of the proximal E-cadherin promoter that contains the identified ZNF217 CRS in vivo. Using a combination of transient transfections and small interfering RNA, we demonstrate that ZNF217 represses the E-cadherin promoter. Collectively, our results implicate ZNF217 and its associated proteins in a novel pathway that may have profound effects on cancer progression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/ZNF217 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0950-9232
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3378-86
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17130829-Base Sequence,
pubmed-meshheading:17130829-Binding Sites,
pubmed-meshheading:17130829-Cadherins,
pubmed-meshheading:17130829-Cell Line, Tumor,
pubmed-meshheading:17130829-Consensus Sequence,
pubmed-meshheading:17130829-DNA,
pubmed-meshheading:17130829-DNA-Binding Proteins,
pubmed-meshheading:17130829-Down-Regulation,
pubmed-meshheading:17130829-Humans,
pubmed-meshheading:17130829-Molecular Sequence Data,
pubmed-meshheading:17130829-Promoter Regions, Genetic,
pubmed-meshheading:17130829-Protein Binding,
pubmed-meshheading:17130829-Sequence Alignment,
pubmed-meshheading:17130829-Trans-Activators,
pubmed-meshheading:17130829-Transcription, Genetic
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pubmed:year |
2007
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pubmed:articleTitle |
Biochemical characterization of the zinc-finger protein 217 transcriptional repressor complex: identification of a ZNF217 consensus recognition sequence.
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pubmed:affiliation |
Department of Oncology, London Regional Cancer Program, The University of Western Ontario, London, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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