Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1991-8-20
|
pubmed:abstractText |
The protective immune response to infection with Chlamydia trachomatis is associated with antibody reactivity to serovar-specific determinants on the major outer membrane protein (MOMP). Because this immunity is T cell dependent, it is essential to define those Th cell determinants that promote natural boosting of the protective antibody response. The gene for MOMP of serovar B was separated into nine overlapping fragments that represent the five C and four V regions. These fragments were expressed as fusion peptides with GST and used to identify the regions of the MOMP that contain T cell determinants recognized in BALB/c mice. We identified peptides that elicit a T cell response to Chlamydia by immunizing mice with the fusion peptides and testing the proliferative response of T cells in vitro to intact organism. For analysis of determinants seen after infection, animals were inoculated with live organism and the T cell proliferative response to each fusion peptide was measured in vitro. In contrast to proliferative analysis in which several regions of the MOMP elicited T cell responses, functional analysis demonstrated that a single fusion peptide, containing V segment three, elicited T cell help in vivo for the production of high titered antisera, specific for protective determinants on the MOMP.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
AIM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0022-1767
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
147
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
674-9
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:1712817-Animals,
pubmed-meshheading:1712817-Antigens, Bacterial,
pubmed-meshheading:1712817-Bacterial Outer Membrane Proteins,
pubmed-meshheading:1712817-Base Sequence,
pubmed-meshheading:1712817-Chlamydia trachomatis,
pubmed-meshheading:1712817-Epitopes,
pubmed-meshheading:1712817-Lymphocyte Activation,
pubmed-meshheading:1712817-Mice,
pubmed-meshheading:1712817-Mice, Inbred BALB C,
pubmed-meshheading:1712817-Molecular Sequence Data,
pubmed-meshheading:1712817-Oligonucleotides,
pubmed-meshheading:1712817-Peptides,
pubmed-meshheading:1712817-Recombinant Fusion Proteins,
pubmed-meshheading:1712817-T-Lymphocytes,
pubmed-meshheading:1712817-T-Lymphocytes, Helper-Inducer
|
pubmed:year |
1991
|
pubmed:articleTitle |
A single peptide from the major outer membrane protein of Chlamydia trachomatis elicits T cell help for the production of antibodies to protective determinants.
|
pubmed:affiliation |
Department of Biomedical and Environmental Health Sciences, University of California, Berkeley 94720.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|