Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-8-19
pubmed:abstractText
Biochemical, pharmacological and immunocytochemical studies have implicated the microtubule-activated ATPase, kinesin, in the movement of membrane bounded organelles in fast axonal transport. In vitro studies suggested that kinesin moves organelles preferentially in the anterograde direction, but data about the function and precise localization of kinesin in the living axon were lacking. The current study was undertaken to establish whether kinesin associates with anterograde or retrograde moving organelles in vivo. Peripheral nerves were ligated to produce accumulations of organelles moving in defined directions. Regions proximal (anterograde) and distal (retrograde) to the ligation were analyzed for kinesin localization by immunofluorescence, and by immunogold electron microscopy using ultracryomicrotomy. Substantial amounts of kinesin were associated with anterograde moving organelles on the proximal side, while significantly less kinesin was detected distally. Statistical analyses indicated that kinesin was mostly associated with membrane-bounded organelles. These observations indicate that axonal kinesin is primarily associated with anterograde moving organelles in vivo.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-1689058, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-1692515, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2143999, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2146510, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2409096, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2415536, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2416467, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2419517, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2466291, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2481499, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2493586, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2497994, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2522351, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2522353, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2525563, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2536030, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2946042, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2955228, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2958482, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2959372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-2965791, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-3102475, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-3117808, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-3134048, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-3141070, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-3926325, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-4399631, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-6153657, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-6159657, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-6162922, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-6181077, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-6425303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1712789-7440248
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
295-302
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Kinesin associates with anterogradely transported membranous organelles in vivo.
pubmed:affiliation
Department of Anatomy and Cell Biology, University of Tokyo, School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't