| pubmed-article:1712550 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0005961 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0018956 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0026032 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:1712550 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:1712550 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1712550 | pubmed:dateCreated | 1991-8-13 | lld:pubmed |
| pubmed-article:1712550 | pubmed:abstractText | Complete yet nontoxic removal of tumor cells from autologous marrow grafts has proved difficult. New methods for separating normal stem cells from tumor cells are needed. The CD34+ cells in bone marrow, 1-2% of the low-density leukocytes, include precursors of all lymphohematopoietic lineages and probably also the primitive cells responsible for engraftment. A nontoxic, inexpensive, reproducible, and clinically applicable method for positive selection of CD34+ cells was developed. Paramagnetic microspheres coated with goat anti-mouse IgG1 are used to partition the cells; brief incubation with chymopapain is used to release them from the beads. Chymopapain exposure does not injury colony-forming cells or delay engraftment in rodents. Clinical volumes of bone marrow can be processed rapidly. In pilot experiments, the resulting grafts have a purity of 85-99% CD34+ cells and 40% median recovery of the assayable colony-forming cells. These studies form the background for a Phase I trial of autologous BMT using CD34+ stem cells. | lld:pubmed |
| pubmed-article:1712550 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1712550 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1712550 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1712550 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1712550 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1712550 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1712550 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1712550 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1712550 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1712550 | pubmed:issn | 0192-8562 | lld:pubmed |
| pubmed-article:1712550 | pubmed:author | pubmed-author:CivinC ICI | lld:pubmed |
| pubmed-article:1712550 | pubmed:author | pubmed-author:RowleyS DSD | lld:pubmed |
| pubmed-article:1712550 | pubmed:author | pubmed-author:StraussL CLC | lld:pubmed |
| pubmed-article:1712550 | pubmed:author | pubmed-author:WinerL DLD | lld:pubmed |
| pubmed-article:1712550 | pubmed:author | pubmed-author:TrischmannT... | lld:pubmed |
| pubmed-article:1712550 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1712550 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:1712550 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1712550 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1712550 | pubmed:pagination | 217-21 | lld:pubmed |
| pubmed-article:1712550 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:meshHeading | pubmed-meshheading:1712550-... | lld:pubmed |
| pubmed-article:1712550 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1712550 | pubmed:articleTitle | Selection of normal human hematopoietic stem cells for bone marrow transplantation using immunomagnetic microspheres and CD34 antibody. | lld:pubmed |
| pubmed-article:1712550 | pubmed:affiliation | Division of Pediatric Oncology, Johns Hopkins Oncology Center, Baltimore, Maryland. | lld:pubmed |
| pubmed-article:1712550 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1712550 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1712550 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1712550 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1712550 | lld:pubmed |
