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rdf:type |
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lifeskim:mentions |
umls-concept:C0014834,
umls-concept:C0035553,
umls-concept:C0035696,
umls-concept:C0181496,
umls-concept:C0205145,
umls-concept:C0206243,
umls-concept:C0243071,
umls-concept:C1522485,
umls-concept:C1552961,
umls-concept:C1705425,
umls-concept:C1705483
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pubmed:issue |
8
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pubmed:dateCreated |
1991-8-14
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pubmed:abstractText |
mRNA analogues approximately 40 bases long were prepared by T7 transcription from synthetic DNA templates. Each message contained the sequence ACC-GCG (coding for threonine and alanine, respectively), together with a single thio-U residue located at a variable position on the 3'-side of these coding triplets. The thio-U residue was either substituted with 4-azidophenacyl bromide to introduce a photo-reactive group, or was left unsubstituted for direct UV cross-linking. After binding to Escherichia coli 70S ribosomes in the presence of tRNA-Thr or tRNA-Ala, the thio-U residue or azidophenyl group was photo-activated and the products of cross-linking (which was exclusively to the 30S subunit) were analysed. Immunological analysis of the cross-linked proteins showed that S5 and S3, together with S1, were the targets of cross-linking at positions close to the decoding site, with the cross-linking to S3 and S1 persisting at positions further away. Analysis of the 16S RNA showed cross-links to the region of bases 1390-1400 in all cases, but in one instance (with the reactive nucleotide 11 bases from the decoding site) simultaneous cross-linking was observed to the latter region and to position 532; these two RNA regions are far apart in current three-dimensional models of the 30S subunit.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-11584834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-1237309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-1694177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-1697060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-1702198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2156079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2182126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2251116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2405392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2422386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2430725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2434656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2443708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2451022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2464693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2468130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2473436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2645171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2646595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2690012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-2953976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3049604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3054810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3071669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3278299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3283702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3288761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3350817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-360171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-384003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-3845122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-409999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-4129206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-6179936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-6381487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-6813860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-692735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1712292-7029211
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Ala,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Transfer, Thr,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2195-202
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1712292-Amino Acid Sequence,
pubmed-meshheading:1712292-Base Sequence,
pubmed-meshheading:1712292-Cross-Linking Reagents,
pubmed-meshheading:1712292-DNA, Bacterial,
pubmed-meshheading:1712292-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1712292-Escherichia coli,
pubmed-meshheading:1712292-Molecular Sequence Data,
pubmed-meshheading:1712292-Photochemistry,
pubmed-meshheading:1712292-RNA, Bacterial,
pubmed-meshheading:1712292-RNA, Messenger,
pubmed-meshheading:1712292-RNA, Ribosomal, 16S,
pubmed-meshheading:1712292-RNA, Transfer, Ala,
pubmed-meshheading:1712292-RNA, Transfer, Thr,
pubmed-meshheading:1712292-RNA-Directed DNA Polymerase,
pubmed-meshheading:1712292-Ribosomes,
pubmed-meshheading:1712292-Templates, Genetic
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pubmed:year |
1991
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pubmed:articleTitle |
The path of mRNA through the Escherichia coli ribosome; site-directed cross-linking of mRNA analogues carrying a photo-reactive label at various points 3' to the decoding site.
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pubmed:affiliation |
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, FRG.
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pubmed:publicationType |
Journal Article
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