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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1991-8-15
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pubmed:abstractText |
Decay-accelerating factor (DAF) was purified from human pooled urine by conventional techniques. The urine DAF was separated into two peaks, pool I and pool II, by gel chromatography. DAF-U1 was isolated from pool I by hydrophobic chromatography, and DAF-U2 from pool II by anti-DAF IgG column. The specific activities of DAF-U1 and DAF-U2 to decay membrane-phase C5 convertase were about 3% and 70% of membrane form DAF, respectively. However, both urine DAFs revealed a similar activity to each other and slightly higher activity than that of membrane form DAF in decay-accelerating fluid-phase C3 convertase of the alternative pathway.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
1074
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
326-30
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:1712233-Amino Acid Sequence,
pubmed-meshheading:1712233-Antigens, CD55,
pubmed-meshheading:1712233-Chromatography, Affinity,
pubmed-meshheading:1712233-Chromatography, Gel,
pubmed-meshheading:1712233-Complement C3-C5 Convertases,
pubmed-meshheading:1712233-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1712233-Humans,
pubmed-meshheading:1712233-Membrane Proteins,
pubmed-meshheading:1712233-Molecular Sequence Data
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pubmed:year |
1991
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pubmed:articleTitle |
Isolation of two forms of decay-accelerating factor (DAF) from human urine.
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pubmed:affiliation |
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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