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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
1991-8-5
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pubmed:abstractText |
Bovine rotavirus proteins were analysed by a panel of monoclonal antibodies. Glycosylated epitopes were identified on both inner and outer capsid proteins (VP6 and VP7 respectively). VP7 possessed a periodate insensitive epitope which was, however, sensitive to endoglycosidase H, mixed glycosidases and to protease treatment. This epitope was not detected on viruses grown in the presence of 2-deoxy-D-glucose or tunicamycin. An epitope was detected on VP6 which was sensitive to periodate oxidation. The blotted protein reacted with a glycan assay kit; yet the epitope was not affected by endoglycosidase H and was found on viruses grown in the presence of 2-deoxy-D-glucose or tunicamycin. These results suggest that VP7 and VP6 epitopes are carbohydrate dependent. The VP7 epitope contains an N-linked carbohydrate moiety in contrast to the VP6 epitope which appears to contain O-linked glycosyl units.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylglucosaminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyglucose,
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoside Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Mannosyl-Glycoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/Periodic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Tunicamycin,
http://linkedlifedata.com/resource/pubmed/chemical/VP6 protein, Rotavirus,
http://linkedlifedata.com/resource/pubmed/chemical/VP7 protein, Rotavirus,
http://linkedlifedata.com/resource/pubmed/chemical/metaperiodate
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-51
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1711988-Acetylglucosaminidase,
pubmed-meshheading:1711988-Animals,
pubmed-meshheading:1711988-Antibodies, Monoclonal,
pubmed-meshheading:1711988-Antigens, Viral,
pubmed-meshheading:1711988-Capsid,
pubmed-meshheading:1711988-Capsid Proteins,
pubmed-meshheading:1711988-Cattle,
pubmed-meshheading:1711988-Deoxyglucose,
pubmed-meshheading:1711988-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1711988-Endopeptidases,
pubmed-meshheading:1711988-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1711988-Epitopes,
pubmed-meshheading:1711988-Glycoproteins,
pubmed-meshheading:1711988-Glycoside Hydrolases,
pubmed-meshheading:1711988-Glycosylation,
pubmed-meshheading:1711988-Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase,
pubmed-meshheading:1711988-Oxidation-Reduction,
pubmed-meshheading:1711988-Periodic Acid,
pubmed-meshheading:1711988-Precipitin Tests,
pubmed-meshheading:1711988-Rotavirus,
pubmed-meshheading:1711988-Tunicamycin
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pubmed:year |
1991
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pubmed:articleTitle |
Characterization of bovine rotavirus VP6 and VP7 as glycoproteins using monoclonal antibodies.
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pubmed:affiliation |
Virology Section, Faculty of Veterinary Medicine, University of Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article
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