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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
47
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|
pubmed:dateCreated |
2006-11-22
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|
pubmed:abstractText |
The first effective method for the introduction of a versatile substituent on 3-phenyl-3-trifluoromethyldiazirine has been developed. The simple preparation of a useful aldehyde intermediate allows easy access to various elaborated photoaffinity ligands, including a l-phenylalanine analog bearing a diazirine ring (TmdPhe). The asymmetric synthesis of TmdPhe was easily accomplished in gram quantities. Site-directed incorporation of this compound into the structure of a calmodulin-binding peptide using automated peptide synthesis afforded a photoreactive peptide that was successfully used for the specific labeling of calmodulin.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Nov
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|
pubmed:issn |
0002-7863
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
29
|
|
pubmed:volume |
128
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
15092-3
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pubmed:dateRevised |
2011-11-17
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|
pubmed:meshHeading |
|
|
pubmed:year |
2006
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|
pubmed:articleTitle |
Simple and versatile method for tagging phenyldiazirine photophores.
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|
pubmed:affiliation |
Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
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|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
