Linked Life Data

17114448

Source:http://linkedlifedata.com/resource/pubmed/id/17114448

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2006-11-20
pubmed:abstractText
Conflicting findings have recently been presented as to the sites and sources of B cells that undergo class switch recombination (CSR) to IgA in the gut. In this study we provide compelling evidence in CD40(-/-) mice demonstrating that IgA CSR can be independent of CD40 signaling and germinal center formation and does not occur in the gut lamina propria (LP) itself. We found that CD40(-/-) mice had near normal levels of gut total IgA despite lacking germinal centers and completely failing to raise specific responses against the T cell-dependent Ags cholera toxin and keyhole limpet hemocyanin. The Peyer's patches in CD40(-/-) mice expressed unexpectedly high levels of activation-induced cytidine deaminase mRNA and germline alpha transcripts, but few postswitch circular DNA transcripts, arguing against significant IgA CSR. Moreover and more surprisingly, wild-type mice exhibited no to low IgA CSR in mesenteric lymph nodes or isolated lymphoid follicles. Importantly, both strains failed to demonstrate any of the molecular markers for IgA CSR in the gut LP itself. Whereas all of the classical sites for IgA CSR in the GALT in CD40(-/-) mice appeared severely compromised for IgA CSR, B cells in the peritoneal cavity demonstrated the expression of activation-induced cytidine deaminase mRNA comparable to that of wild-type mice. However, peritoneal cavity B cells in both strains expressed intermediate levels of the germinal center marker GL7 and exhibited no germline alpha transcripts, and only three of 51 mice analyzed showed the presence of postswitch circular DNA transcripts. Taken together, these findings strongly argue for alternative inductive sites for gut IgA CSR against T cell-independent Ags outside of the GALT and the nonorganized LP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7772-83
pubmed:meshHeading
pubmed-meshheading:17114448-Animals, pubmed-meshheading:17114448-Antigens, CD40, pubmed-meshheading:17114448-Antigens, Differentiation, pubmed-meshheading:17114448-Blotting, Southern, pubmed-meshheading:17114448-Cytidine Deaminase, pubmed-meshheading:17114448-DNA, Circular, pubmed-meshheading:17114448-DNA Primers, pubmed-meshheading:17114448-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:17114448-Flow Cytometry, pubmed-meshheading:17114448-Germinal Center, pubmed-meshheading:17114448-Immunity, Mucosal, pubmed-meshheading:17114448-Immunoglobulin A, pubmed-meshheading:17114448-Immunoglobulin Class Switching, pubmed-meshheading:17114448-Immunohistochemistry, pubmed-meshheading:17114448-Intestines, pubmed-meshheading:17114448-Lymph Nodes, pubmed-meshheading:17114448-Mice, pubmed-meshheading:17114448-Mice, Inbred C57BL, pubmed-meshheading:17114448-Peyer's Patches, pubmed-meshheading:17114448-RNA, Messenger, pubmed-meshheading:17114448-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2006
pubmed:articleTitle
Gut IgA class switch recombination in the absence of CD40 does not occur in the lamina propria and is independent of germinal centers.
pubmed:affiliation
Department of Microbiology and Immunology, Mucosal Immunobiology and Vaccine Research Center, Institute of Biomedicine, Göteborg University, 405 30 Göteborg, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't