Source:http://linkedlifedata.com/resource/pubmed/id/17114420
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2006-11-20
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pubmed:abstractText |
The Ubc13 E2 ubiquitin-conjugating enzyme is essential for BCR-, TLR-, and IL-1 receptor (IL-1R)-mediated immune responses. Although Ubc13-deficient mice show defects in BCR-, TLR/IL-1R-, or CD40-mediated activation of mitogen-activated protein kinases, the function of Ubc13 in TCR-mediated signaling and responses remains uncertain. To address this, we here generated T cell-specific conditional Ubc13-deficient mice. The frequency of T lymphocytes was severely reduced in spleens from Ubc13-deficient mice. Moreover, Ubc13-deficient thymocytes displayed defective proliferation in response to anti-CD3/CD28 or PMA/ionophore stimulation. Regarding the signal transduction, although NF-kappaB activation was modestly affected, PMA/ionophore-induced activation of Jnk and p38 was profoundly impaired in Ubc13-deficient thymocytes. In addition, PMA/ionophore-mediated ubiquitination of NF-kappaB essential modulator (NEMO)/IkappaB kinase gamma (IKKgamma) and phosphorylation of TGF-beta-activated kinase 1 (TAK1) were nearly abolished in Ubc13-deficient thymocytes. Thus, Ubc13 plays an important role in thymocyte TCR-mediated signaling and immune responses.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Ubc13 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Conjugating Enzymes
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7520-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17114420-Animals,
pubmed-meshheading:17114420-Blotting, Western,
pubmed-meshheading:17114420-Cell Proliferation,
pubmed-meshheading:17114420-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:17114420-Enzyme Activation,
pubmed-meshheading:17114420-Flow Cytometry,
pubmed-meshheading:17114420-Immunoprecipitation,
pubmed-meshheading:17114420-Lymphocyte Activation,
pubmed-meshheading:17114420-Mice,
pubmed-meshheading:17114420-Mice, Transgenic,
pubmed-meshheading:17114420-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:17114420-NF-kappa B,
pubmed-meshheading:17114420-Receptors, Antigen, T-Cell,
pubmed-meshheading:17114420-Signal Transduction,
pubmed-meshheading:17114420-T-Lymphocytes,
pubmed-meshheading:17114420-Ubiquitin-Conjugating Enzymes
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pubmed:year |
2006
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pubmed:articleTitle |
Cutting Edge: Pivotal function of Ubc13 in thymocyte TCR signaling.
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pubmed:affiliation |
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0851, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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