Linked Life Data

17113082

Source:http://linkedlifedata.com/resource/pubmed/id/17113082

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28-29
pubmed:dateCreated
2006-12-4
pubmed:abstractText
We investigated the effect of target mRNA level on grafting-transmitted gene silencing in tomato plants by using a strong ACC oxidase 1 (ACO1) silencer as the stock and transgenic ACO1 overexpressers as scions. Manifestation of graft transmission of sense gene silencing required a high initial level of target mRNA in the scion. A relatively high level of siRNA, similar to that in the strong ACO1 silencer, was also detected in the silencing-susceptible strong ACO1 overexpressers prior to grafting. After grafting the silencing signal from the stock enhanced the level of the siRNAs in the scion and the ACO1 mRNA level was reduced dramatically. Using stock and scions producing different siRNAs we provided evidence that the transmissible silencing signal does not correspond to the bulk siRNAs in the stock. We also showed, contrary to a previous report, that antisense silencing was graft-transmissible but it took longer to manifest itself. The delay in graft transmission from antisense-silenced plants could be attributed to the difference in the nature or strength of the signal or the mechanism of its amplification, but is further evidence of mechanistic similarities between sense and antisense silencing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
580
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6579-86
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
The mechanism of graft transmission of sense and antisense gene silencing in tomato plants.
pubmed:affiliation
Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't