Linked Life Data

17111624

Source:http://linkedlifedata.com/resource/pubmed/id/17111624

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-11-20
pubmed:abstractText
Based on a screen printed carbon electrode which contained a sensitive film prepared by crosslinking acetylcholine esterase (AChE) with glutaraldehyde on the surface of working electrode, an amperometric enzyme biosensor was taken on the determination of organophosphorus (OP) pesticide in water environment. Cyclic voltammetry was applied to assess the coherence of prepared screen printed electrodes. The results show that the variation is less than 10%. The sensor sensitivity was optimized with respect to content of enzyme, crosslinker and substrate. The best sensitivity is obtained using 0.2% of glutaraldehyde, 0.5 mg/mL of AChE and 10 mmol/L of acetylthiocholine chloride (ATChCl). Amperometric I-t curve was conducted to detect the decrease rate of the current after the addition of two kind of OPs (terbufos and parathion), respectively. The detection limit for two kind of OPs are found to be 1 ng/mL with the linear range 1 ng/mL - 10 000 ng/mL. Cyclic voltammetry was conducted to detect parathion on the sensors which immobilized AChE by physical adsorption. The results show that it can achieve a better sensitivity than amperometric I-t curve.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0250-3301
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1627-30
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
[Detecting organophosphorus pesticide in water environment using an enzyme biosensor].
pubmed:affiliation
Environmental Simulation and Pollution Control State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China. chenxq99@mails.tsinghua.edu.cn
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't